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Hif1ab antibody

Anti-Hif1ab antibody used in Western Blot (WB). GTX131826

Cat No. GTX131826

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Zebrafish
Package
200 μl ($389),
50 μl ($199)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Positive Control

zebrafish embryos (untreated) , zebrafish embryos (treated UV irradiation)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of zebrafish Hif1ab. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

hypoxia inducible factor 1 subunit alpha b,hif1a,wu:fb63a10,zgc:55405

Database

Research Area

DATA IMAGES
Anti-Hif1ab antibody used in Western Blot (WB). GTX131826

GTX131826 WB Image

Hif1ab antibody detects Hif1ab protein by western blot analysis. Un-treated (-) and treated (+, UVC treatment for ) zebrafish embryos whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hif1ab antibody (GTX131826) at a dilution of 1:1000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199