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Histamine H2 Receptor antibody [N1], N-term

Anti-Histamine H2 Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108152
Anti-Histamine H2 Receptor antibody [N1], N-term used in Western Blot (WB). GTX108152
Anti-Histamine H2 Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108152

Cat. No. GTX108152

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IHC

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Histamine H2 Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human Histamine H2 Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037202

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histamine receptor H2 , H2R , HH2R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histamine H2 Receptor antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108152

GTX108152 ICC/IF Image

Histamine H2 Receptor antibody [N1], N-term detects Histamine H2 Receptor protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histamine H2 Receptor protein stained by Histamine H2 Receptor antibody [N1], N-term (GTX108152) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Histamine H2 Receptor antibody [N1], N-term used in Western Blot (WB). GTX108152

GTX108152 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Histamine H2 Receptor antibody [N1], N-term (GTX108152) diluted at 1:1000.

Anti-Histamine H2 Receptor antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX108152

GTX108152 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H520 xenograft, using Histamine H2 Receptor(GTX108152) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Crouchet E et al. Nat Commun 2021; 12 (1) : 5525 A human liver cell-based system modeling a clinical prognostic liver signature for therapeutic discovery.
Application : IHC-P
Reactivity : Human
Liu X et al. Eur J Pharmacol 2017; 810 : 156-162 Suppression of osteogenic differentiation in mesenchymal stem cells from patients with ossification of the posterior longitudinal ligament by a histamine-2-receptor antagonist.
Application : WB
Reactivity : Human
Adderley SP et al. Microcirculation 2015; 22 (4) : 237-48 Involvement of the H1 Histamine Receptor, p38 MAP Kinase, Myosin Light Chains Kinase, and Rho/ROCK in Histamine-Induced Endothelial Barrier Dysfunction.
Kurtz KH et al. Microcirculation 2014; Involvement of H1 and H2 receptors and soluble guanylate cyclase in histamine-induced relaxation of rat mesenteric collecting lymphatics.
Application : WB, IHC
Reactivity : Rat
Frei R et al. J Allergy Clin Immunol 2013; 132 (1) : 194-204 Histamine receptor 2 modifies dendritic cell responses to microbial ligands.
Application : FACS
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169