GeneTex

  • Country / Location Selection

United States (US)

Histamine H3 Receptor antibody

Anti-Histamine H3 Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100187
Anti-Histamine H3 Receptor antibody used in Western Blot (WB). GTX100187

Cat. No. GTX100187

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Histamine H3 Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

A431 , H1299

Predict Reactivity

Mouse, Rat, Guinea pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Histamine H3 Receptor. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1080985

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histamine receptor H3 , GPCR97 , HH3R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histamine H3 Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100187

GTX100187 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using Histamine H3 Receptor(GTX100187) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histamine H3 Receptor antibody used in Western Blot (WB). GTX100187

GTX100187 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX100187 diluted at 1:1000

REFERENCE

Application Reference

She X et al. Biomed Pharmacother 2021; 140 : 111748 A high-throughput screen identifies inhibitors of lung cancer stem cells.
Application : IHC-P
Reactivity : Human
Yadav M et al. Biochim Biophys Acta 2016; (Epub) epidermal growth factor receptor inhibitor cancer drug * modulates cell growth and differentiation of acute myeloid leukemia cells via histamine receptors.
Application : WB
Reactivity : Human
Adderley SP et al. Microcirculation 2015; 22 (4) : 237-48 Involvement of the H1 Histamine Receptor, p38 MAP Kinase, Myosin Light Chains Kinase, and Rho/ROCK in Histamine-Induced Endothelial Barrier Dysfunction.
Submit a Reference

REVIEW

There are currently no reviews for Histamine H3 Receptor antibody (GTX100187). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169