GeneTex
United States (US)

Histamine H3 Receptor antibody

GTX100187_39399_IHC_19011506_162.jpg
GTX100187_WB_19011506_198.jpg

Cat No. GTX100187

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

A431 , H1299

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histamine H3 Receptor. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GPCR97 antibody, HH3R antibody, HRH3 antibody, G protein-coupled receptor 97 antibody, histamine H3 receptor antibody, H3R antibody, G-protein coupled receptor 97 antibody, histamine receptor H3 antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100187_39399_IHC_19011506_162.jpg

GTX100187 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using Histamine H3 Receptor(GTX100187) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100187_WB_19011506_198.jpg

GTX100187 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGE
GTX100187 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169