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Histamine H4 Receptor antibody [N1N2], N-term

Anti-Histamine H4 Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX100387
Anti-Histamine H4 Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX100387

Cat No. GTX100387

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 50% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histamine H4 Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

histamine receptor H4,AXOR35,BG26,GPCR105,GPRv53,H4,H4R,HH4R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H4 belongs to the family 1 of G protein-coupled receptors and has the highest homology to histamine receptor H3 among known G protein-coupled receptors. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Histamine H4 Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX100387

GTX100387 WB Image

Histamine H4 Receptor antibody detects Histamine H4 Receptor protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
Histamine H4 Receptor antibody (GTX100387) dilution: 1:500

Anti-Histamine H4 Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX100387

GTX100387 WB Image

Histamine H4 Receptor antibody detects Histamine H4 Receptor protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HRH4 shRNA-transfected HeLa cells
10 % SDS-PAGE
Histamine H4 Receptor antibody (GTX100387) dilution: 1:500

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169