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Histamine H4 Receptor antibody [N1N2], N-term

KO/KD
Anti-Histamine H4 Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX100387
Anti-Histamine H4 Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX100387

Cat. No. GTX100387

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Histamine H4 Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , human shHRH4-transfected HeLa cells

Predict Reactivity

Mouse, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Histamine H4 Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240963

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histamine receptor H4 , AXOR35 , BG26 , GPCR105 , GPRv53 , H4 , H4R , HH4R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H4 belongs to the family 1 of G protein-coupled receptors and has the highest homology to histamine receptor H3 among known G protein-coupled receptors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histamine H4 Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX100387

GTX100387 WB Image

KO/KD

Histamine H4 Receptor antibody detects Histamine H4 Receptor protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of HRH4 shRNA-transfected HeLa cells
10 % SDS-PAGE
Histamine H4 Receptor antibody (GTX100387) dilution: 1:500

Anti-Histamine H4 Receptor antibody [N1N2], N-term used in Western Blot (WB). GTX100387

GTX100387 WB Image

Histamine H4 Receptor antibody detects Histamine H4 Receptor protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
Histamine H4 Receptor antibody (GTX100387) dilution: 1:500

REFERENCE

Application Reference

Adderley SP et al. Microcirculation 2015; 22 (4) : 237-48 Involvement of the H1 Histamine Receptor, p38 MAP Kinase, Myosin Light Chains Kinase, and Rho/ROCK in Histamine-Induced Endothelial Barrier Dysfunction.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169