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Histamine H4 Receptor antibody

Anti-Histamine H4 Receptor antibody used in Western Blot (WB). GTX130116

Cat No. GTX130116

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HL-60 MF (unboiled)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human Histamine H4 Receptor.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histamine receptor H4 , AXOR35 , BG26 , GPCR105 , GPRv53 , H4 , H4R , HH4R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H4 belongs to the family 1 of G protein-coupled receptors and has the highest homology to histamine receptor H3 among known G protein-coupled receptors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histamine H4 Receptor antibody used in Western Blot (WB). GTX130116

GTX130116 WB Image

Histamine H4 Receptor antibody detects Histamine H4 Receptor protein by western blot analysis. HL-60 whole cell extracts and membrane extracts (unboiled) (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Histamine H4 Receptor antibody (GTX130116) diluted at 1:1000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169