GeneTex

  • Country / Location Selection

United States (US)

Histamine H4 Receptor antibody

Orthogonal
Anti-Histamine H4 Receptor antibody used in Western Blot (WB). GTX130116

Cat. No. GTX130116

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Histamine H4 Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HL-60 MF (unboiled)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human Histamine H4 Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886183

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histamine receptor H4 , AXOR35 , BG26 , GPCR105 , GPRv53 , H4 , H4R , HH4R

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H4 belongs to the family 1 of G protein-coupled receptors and has the highest homology to histamine receptor H3 among known G protein-coupled receptors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histamine H4 Receptor antibody used in Western Blot (WB). GTX130116

GTX130116 WB Image

Orthogonal

Histamine H4 Receptor antibody detects Histamine H4 Receptor protein by western blot analysis. HL-60 whole cell extracts and membrane extracts (unboiled) (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Histamine H4 Receptor antibody (GTX130116) diluted at 1:1000.

REFERENCE

Application Reference

Yadav M et al. Biochim Biophys Acta 2016; (Epub) epidermal growth factor receptor inhibitor cancer drug * modulates cell growth and differentiation of acute myeloid leukemia cells via histamine receptors.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Histamine H4 Receptor antibody (GTX130116). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169