Histamine antibody

Cat. No. GTX134710

GTX134710 Dot Image

Dot blot analysis of 100 ng BSA-conjugated Histamine, using Histamine antibody (GTX134710) at 1:4000, 1:8000, 1:16000 and 1:32000.

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GTX134710 ELISA Image

ELISA plate is coated with 100 μL of Histamine-carrier protein conjugate at 10 μg/mL. The coated ligand is detected with Histamine antibody (GTX134710) at concentrations ranging from 440 ng/mL down to 1.6 ng/mL. Rabbit IgG antibody (HRP) (GTX213110-01) is diluted at 1:2,000 and used to detect the primary antibody.

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GTX134710 ICC/IF Image

Histamine antibody detects Histamine protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histamine stained by Histamine antibody (GTX134710) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

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This Histamine antibody (GTX134710) is a rabbit polyclonal antibody validated for detection of Histamine by ICC/IF, IHC-Fr, Dot, and ELISA. Reactivity is species-independent.

Host

Rabbit
Clonality

Polyclonal
Isotype

IgG
Applications

ICC/IF IHC-Fr Dot ELISA
Reactivity

Species independent

Summary

Antibody specificity was performed with a competitive ELISA test with the following compounds:

Compounds	Cross-reactivity ratio
Histamine-G-CP	1
Dopamine-G-CP	1/>10,000
Tryptamine-G-CP	1/>10,000

G: Glutaraldehyde, CP: Carrier Protein (Gelatin).