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Histone H1.1 antibody

Orthogonal
Anti-Histone H1.1 antibody used in Western Blot (WB). GTX117055

Cat. No. GTX117055

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Histone H1.1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Observed MW

33 kDa. The observed M.W. is based on the following publication. PMID: 19284784

Positive Control

Jurkat , Jurkat NE

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone H1.1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885410

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone cluster 1 H1 family member a , H1.1 , H1A , H1F1 , HIST1

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H1.1 antibody used in Western Blot (WB). GTX117055

GTX117055 WB Image

Orthogonal

Histone H1.1 antibody detects Histone H1.1 protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H1.1 antibody (GTX117055) diluted by 1:1000.
Observed Histone H1.1 protein is about 33kDa.
Reference: J Proteome Res.?2009 May;8(5):2310-8.

REFERENCE

Application Reference

Ozgencil M et al. Life Sci Alliance 2023; 6 (9) The linker histone H1-BRCA1 axis is a crucial mediator of replication fork stability.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169