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Histone H1.2 antibody, Internal

Anti-Histone H1.2 antibody, Internal used in Western Blot (WB). GTX46484
Anti-Histone H1.2 antibody, Internal used in ChIP assay (ChIP assay). GTX46484

Cat. No. GTX46484

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ChIP assay

Reactivity

 Human
Package
50 μg ($409)
See all Histone H1.2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

human B lymphoblast

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human Histone H1.2.

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone cluster 1 H1 family member c , H1.2 , H1C , H1F2 , H1s-1

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H1.2 antibody, Internal used in Western Blot (WB). GTX46484

GTX46484 WB Image

WB analysis of human B lymphoblast cells using GTX46484 HIST1H1C antibody at 0.2-1μg/ml.

Anti-Histone H1.2 antibody, Internal used in ChIP assay (ChIP assay). GTX46484

GTX46484 ChIP assay Image

ChIP analysis of HCT116 cells using GTX46484 HIST1H1C antibody. Use 0.5μg antibody GTX46484 HIST1H1C antibody for ChIP assay.

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409