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Histone H1.2 antibody

Anti-Histone H1.2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122561
Anti-Histone H1.2 antibody used in IHC (Paraffin sections) (IHC-P). GTX122561
Anti-Histone H1.2 antibody used in Western Blot (WB). GTX122561

Cat No. GTX122561

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HeLa , HeLa nucleus
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone H1.2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

histone cluster 1 H1 family member c,H1.2,H1C,H1F2,H1s-1

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Histone H1.2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122561

GTX122561 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Histone H1.2(GTX122561) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Histone H1.2 antibody used in IHC (Paraffin sections) (IHC-P). GTX122561

GTX122561 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Histone H1.2(GTX122561) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H1.2 antibody used in Western Blot (WB). GTX122561

GTX122561 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HeLa nucleus
12% SDS PAGE
GTX122561 diluted at 1:10000

REFERENCE
REVIEW

Histone H1.2 antibody Cat No. GTX122561

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 25-Jul-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Amount used : 7 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane 1: HEK293 cytoplasmic extract, Lane 2: HEK293 nuclear extract
SDS
Glycerol.pdf
Thimerosal.pdf