APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa Nucleus
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H1.4. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10729585
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
histone cluster 1 H1 family member e , H1.4 , H1E , H1F4 , H1s-4 , RMNS , dJ221C16.5
Cellular Localization
Nucleus
Background
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Histone H1.4 antibody [N1N2], N-term used in Western Blot (WB). GTX121670](/templates/default/js/lightbox/images/loading.gif "Anti-Histone H1.4 antibody [N1N2], N-term used in Western Blot (WB). GTX121670")
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GTX121670 WB Image
Sample (20 ug of cell lysate )
A: HeLa nucleus
12% SDS PAGE
GTX121670 diluted at 1:1000
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GTX121670 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histone H1.4(GTX121670) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for Histone H1.4 antibody [N1N2], N-term (GTX121670). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Histone H1.4 antibody [N1N2], N-term (GTX121670). Be the first to share your experience with this product.
![Anti-Histone H1.4 antibody [N1N2], N-term used in Western Blot (WB). GTX121670](/upload/website/prouct_img/small/GTX121670/GTX121670_40380_WB_18121410_413.jpg "Anti-Histone H1.4 antibody [N1N2], N-term used in Western Blot (WB). GTX121670")
![Anti-Histone H1.4 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121670](/upload/website/prouct_img/small/GTX121670/GTX121670_40380_IHC_18121410_809.jpg "Anti-Histone H1.4 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121670")
![Anti-Histone H1.4 antibody [N1N2], N-term used in Western Blot (WB). GTX121670](/upload/website/prouct_img/normal/GTX121670/GTX121670_40380_WB_18121410_413.jpg "Anti-Histone H1.4 antibody [N1N2], N-term used in Western Blot (WB). GTX121670")
![Anti-Histone H1.4 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121670](/upload/website/prouct_img/normal/GTX121670/GTX121670_40380_IHC_18121410_809.jpg "Anti-Histone H1.4 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX121670")