GeneTex
United States (US)

Histone H1K25ac (acetyl Lys25) antibody

Histone-H1-acetyl-Lys25-antibody-GTX88006-WB-1_18121411_470.jpg
Histone-H1-acetyl-Lys25-antibody-GTX88006-IHC-1_18121411_993.jpg

Cat No. GTX88006

Host Rabbit
Clonality Polyclonal
Application WB, IHC-P, ELISA, IHC
Reactivity Human, Monkey
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

21 kDa. ( Note )

Specificity/Sensitivity

Histone H1 (Acetyl-Lys25) Antibody detects endogenous levels of total Histone H1 protein only when acetylated at lysine25.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H1 around the acetylated site of Lys25.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acet

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

acetyl Histone H1K25ac K25 Antibody , Histone H1K25ac Lys25 acetyl Antibody , acetyl Lys25 Histone H1K25ac Antibody , H1FOO Antibody , Histone H1K25ac acetyl K25 Antibody , Histone H1K25ac K25 acetyl Antibody , Histone H1K25ac acetyl Lys25 Antibody , acetyl K25 Histone H1K25ac Antibody , Histone H1K25ac acetyl Antibody , OSH1 Antibody , acetyl Histone H1K25ac Lys25 Antibody , acetyl Histone H1K25ac Antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The protein encoded is a member of the histone H1 family. The related mouse gene is expressed only in oocytes. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES
Histone-H1-acetyl-Lys25-antibody-GTX88006-WB-1_18121411_470.jpg

GTX88006 WB Image

Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H1 (Acetyl-Lys25) Antibody. The lane on the right is treated with the synthesized peptide.

Histone-H1-acetyl-Lys25-antibody-GTX88006-IHC-1_18121411_993.jpg

GTX88006 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H1 (Acetyl-Lys25) Antibody. The picture on the right is treated with the synthesized peptide.

REFERENCE
Package List Price ($)
$ 349