GeneTex
United States (US)

Histone H2A/H4 (phospho Ser1) antibody [RM216]

GTX33601_20160517_WB_18121410_504.jpg
GTX33601_20160517_ICCIF_18121410_381.jpg
GTX33601_20160517_ELISA_18121410_480.jpg

Cat No. GTX33601

Host Rabbit
Clonality Monoclonal
Clone Name RM216
Isotype IgG
Application WB, ICC/IF, ELISA, Multiplexing
Reactivity Human
APPLICATION

Application Note

Western Blot: 0.5 ug/mL - 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 1 ug/mL.

Specificity/Sensitivity

This antibody reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

An unmodified peptide corresponding to the N-terminus of Histone H3.

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX33601_20160517_WB_18121410_504.jpg

GTX33601 WB Image

Western Blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole. Using RM216 at 0.5 ug/mL, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells.

GTX33601_20160517_ICCIF_18121410_381.jpg

GTX33601 ICC/IF Image

Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H2A/H4 (Ser1) Rabbit mAb RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

GTX33601_20160517_ELISA_18121410_480.jpg

GTX33601 ELISA Image

RM216 specifically reacts to both Histone H2A and H4phosphorylated at Serine 1 (H2AS1p and H4S1p).

Package List Price ($)
$ 349