Histone H2A.X antibody [N1N2], N-term

Cat. No. GTX108297

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($419), 25 μl ($169)

See all Histone H2A.X products

Datasheet
Document

View product citations for antibody GTX108297 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037175

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Database

Gene ID: 3014 H2AFX
UniProt: P16104 H2AFX

Research Area

DATA IMAGES

GTX108297 ICC/IF Image

Histone H2A.X antibody [N1N2], N-term detects Histone H2A.X protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2A.X protein stained by Histone H2A.X antibody [N1N2], N-term (GTX108297) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX108297 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using Histone H2A.x(GTX108297) antibody at 1:1000 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108297 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
15% SDS PAGE
GTX108297 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 419

25 μl

$ 169

Qty