GeneTex

  • Country / Location Selection

United States (US)

Histone H2A.X antibody

Orthogonal
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108272
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in Immunoprecipitation (IP). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

Cat. No. GTX108272

Reference 10
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all Histone H2A.X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

mouse brain , rat brain , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , HCT116 (30uM cisplatin for 24hr)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950472

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human esophagus cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus cancer.
Histone H2A.X antibody (GTX108272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Histone H2A.X antibody (GTX108272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H2A.X antibody (GTX108272) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Histone H2A.X antibody (GTX108272) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H2A.X antibody (GTX108272) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A.X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108272

GTX108272 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Histone H2A.X (GTX108272) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.X antibody used in Immunoprecipitation (IP). GTX108272

GTX108272 IP Image

Histone H2A.X antibody immunoprecipitates H2AFX protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of H2AFX protein by 4 μg Histone H2A.X antibody (GTX108272)
15 % SDS-PAGE
The immunoprecipitated H2AFX protein was detected by Histone H2A.X antibody (GTX108272) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.
A. 50 μg rat brain extract
12% SDS-PAGE
Histone H2A.X antibody (GTX108272) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108272

GTX108272 ICC/IF Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.
A. 50 μg mouse brain extract
12% SDS-PAGE
Histone H2A.X antibody (GTX108272) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

LH Giang et al. J Exp Clin Cancer Res 2023; 42 (1) : 346 Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor
Application : WB
Reactivity : Human
Thirunavukkarasu S et al. J Clin Invest 2023; 133 (12) Poly(ADP-ribose) polymerase 9 mediates early protection against Mycobacterium tuberculosis infection by regulating type I IFN production.
Liu X et al. Mol Cancer Ther 2019; 18 (12) : 2283-2295 CNDAC-Induced DNA Double-Strand Breaks Cause Aberrant Mitosis Prior to Cell Death.
Lee CY et al. Nat Commun 2019; 10 (1) : 65 Promotion of homology-directed DNA repair by polyamines.
Application : WB
Reactivity : Human
Yu CY et al. Eur J Pharmacol 2017; (Epub) Ovatodiolide isolated from Anisomeles indica induces cell cycle G2/M arrest and apoptosis via a ROS-dependent ATM/ATR signaling pathways.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Histone H2A.X antibody Cat. No. GTX108272

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Date : Anonymous submitted on 04-Jul-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : SUM159 cells with/without treatment
Amount used : 15μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Date : Anonymous submitted on 13-May-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : 4T1 cells
Amount used : 20μg
Blocking : 4% non-fat milk in TBST, 23°C, 1Hr
Primary Antibody : 1:4000, 4°C, 16Hr
Submit a Review
Application Reference
LH Giang et al. J Exp Clin Cancer Res 2023; 42 (1):346 Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor
Application : WB
Reactivity : Human
Thirunavukkarasu S et al. J Clin Invest 2023; 133 (12) Poly(ADP-ribose) polymerase 9 mediates early protection against Mycobacterium tuberculosis infection by regulating type I IFN production.
Liu X et al. Mol Cancer Ther 2019; 18 (12):2283-2295 CNDAC-Induced DNA Double-Strand Breaks Cause Aberrant Mitosis Prior to Cell Death.
Lee CY et al. Nat Commun 2019; 10 (1):65 Promotion of homology-directed DNA repair by polyamines.
Application : WB
Reactivity : Human
Yu CY et al. Eur J Pharmacol 2017; (Epub) Ovatodiolide isolated from Anisomeles indica induces cell cycle G2/M arrest and apoptosis via a ROS-dependent ATM/ATR signaling pathways.
Application : WB
Reactivity : Human
Liu MK et al. Int J Mol Sci 2016; 17 (6) Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response.
Application : WB
Reactivity : Human
Peraldo-Neia C et al. BMC Cancer 2014; 14:918 Anti-cancer effect and gene modulation of ET-743 in human biliary tract carcinoma preclinical models.
Application : WB
Reactivity : Human
Chinnam M et al. J Natl Cancer Inst 2014; 106 (11):pii: dju306 The Thoc1 ribonucleoprotein and prostate cancer progression.
Application : WB
Yuan SS et al. J Natl Cancer Inst 2012; 104 (19):1485-502 Role of MRE11 in cell proliferation, tumor invasion, and DNA repair in breast cancer.
Application : ICC/IF
Reactivity : Human
Chen HM et al. Free Radic Biol Med 2011; 50 (9):1151-62 A novel synthetic protoapigenone analogue, WYC02-9, induces DNA damage and apoptosis in DU145 prostate cancer cells through generation of reactive oxygen species.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169