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Histone H2A.X antibody

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272
Anti-Histone H2A.X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108272
Anti-Histone H2A.X antibody used in Immunoprecipitation (IP). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272
Anti-Histone H2A.X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108272

Cat No. GTX108272

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , HepG2 , HCT116 , HCT116 (30uM cisplatin for 24hr) , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , mouse brain , rat brain , PC-12 , Rat2 , *4T1

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H2A histone family member X,H2A.X,H2A/X,H2AX

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human esophagus cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus cancer.
Histone H2A.X antibody (GTX108272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H2A.X antibody (GTX108272) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Histone H2A.X antibody (GTX108272) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H2A.X antibody (GTX108272) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A.X antibody used in IHC (Paraffin sections) (IHC-P). GTX108272

GTX108272 IHC-P Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Histone H2A.X antibody (GTX108272) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A.X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108272

GTX108272 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using Histone H2A.X (GTX108272) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Histone H2A.X antibody used in Immunoprecipitation (IP). GTX108272

GTX108272 IP Image

Histone H2A.X antibody immunoprecipitates H2AFX protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 40 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of H2AFX protein by 4 μg Histone H2A.X antibody (GTX108272)
15 % SDS-PAGE
The immunoprecipitated H2AFX protein was detected by Histone H2A.X antibody (GTX108272) diluted at 1:2000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.
A. 50 µg mouse brain extract
12% SDS-PAGE
Histone H2A.X antibody (GTX108272) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Histone H2A.X antibody detects Histone H2A.X protein by western blot analysis.
A. 50 µg rat brain extract
12% SDS-PAGE
Histone H2A.X antibody (GTX108272) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX108272

GTX108272 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX108272) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108272

GTX108272 ICC/IF Image

Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

REFERENCE
REVIEW

Histone H2A.X antibody Cat No. GTX108272

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 13-May-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : 4T1 cells
Amount used : 20μg
Blocking : 4% non-fat milk in TBST, 23°C, 1Hr
Primary Antibody : 1:4000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169