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Histone H2A.X antibody

Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343
Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343
Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343

Cat No. GTX127343

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Wm

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

3dpf zebrafish embryos , 3dpf zebrafish embryos (untreated) , 3dpf zebrafish embryos (treated w/wo UV irradiation)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of zebrafish Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H2A histone family, member X , zgc:56329

Cellular Localization

Nucleus

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Database

Research Area

DATA IMAGES

Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343

GTX127343 IHC-Wm Image

Histone H2A.X antibody detects Histone H2A.X protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Histone H2A.X antibody (GTX127343) dilution: 1:100.

Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343

GTX127343 IHC-Wm Image

Histone H2A.X antibody detects Histone H2A.X protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Histone H2A.X antibody (GTX127343) dilution: 1:100.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343

GTX127343 WB Image

Histone H2A.X antibody detects Histone H2A.X protein by Western blot analysis. Un-treated (-) and treated (+, 8000J/m2 UVC treatment) 3dpf zebrafish embryos whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX127343) at a dilution of 1:1000.

Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343

GTX127343 IHC-Wm Image

H2afx antibody detects H2afx protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
H2afx antibody (GTX127343) dilution: 1:200.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343

GTX127343 WB Image

Zebrafish embryos extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX127343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219