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Histone H2A.X antibody

Orthogonal
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343
Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343
Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343
Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343

Cat. No. GTX127343

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish, Nothobranchius furzeri
Package
100 μl ($399),
25 μl ($169)
See all Histone H2A.X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

3dpf zebrafish embryos (treated w/wo UV irradiation) , 3dpf zebrafish embryos

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of zebrafish Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885643

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family, member X , zgc:56329

Cellular Localization

Nucleus

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Database

Research Area

DATA IMAGES

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343

GTX127343 WB Image

Orthogonal

Histone H2A.X antibody detects Histone H2A.X protein by Western blot analysis. Un-treated (-) and treated (+, 8000J/m2 UVC treatment) 3dpf zebrafish embryos whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX127343) at a dilution of 1:1000.

Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343

GTX127343 IHC-Wm Image

Histone H2A.X antibody detects Histone H2A.X protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Histone H2A.X antibody (GTX127343) dilution: 1:100.

Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343

GTX127343 WB Image

Zebrafish embryos extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX127343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343

GTX127343 IHC-Wm Image

Histone H2A.X antibody detects Histone H2A.X protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Histone H2A.X antibody (GTX127343) dilution: 1:100.

Anti-Histone H2A.X antibody used in IHC (Whole mount) (IHC-Wm). GTX127343

GTX127343 IHC-Wm Image

H2afx antibody detects H2afx protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
H2afx antibody (GTX127343) dilution: 1:200.
Anti-Histone H2A.X antibody used in Western Blot (WB). GTX127343

GTX127343 WB Image

The data was published in the journal Cells in 2019.PMID: 31569376

REFERENCE

Application Reference

Cencioni C et al. Cells 2019; 8 (10) Aging Triggers H3K27 Trimethylation Hoarding in the Chromatin of Nothobranchius furzeri Skeletal Muscle.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169