H2afx antibody detects H2afx protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
H2afx antibody (GTX127343) dilution: 1:200.

Histone H2A.X antibody detects Histone H2A.X protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Histone H2A.X antibody (GTX127343) dilution: 1:100.

Zebrafish embryos extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX127343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Histone H2A.X antibody detects Histone H2A.X protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Histone H2A.X antibody (GTX127343) dilution: 1:100.

Histone H2A.X antibody detects Histone H2A.X protein by Western blot analysis. Un-treated (-) and treated (+, 8000J/m2 UVC treatment) 3dpf zebrafish embryos whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody (GTX127343) at a dilution of 1:1000.

The data was published in the journal Cells in 2019.PMID: 31569376