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Histone H2A.XS139ph (phospho Ser139) antibody [3F2]

Orthogonal
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80694
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in Western Blot (WB). GTX80694
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in IHC (Paraffin sections) (IHC-P). GTX80694
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80694
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80694

Cat. No. GTX80694

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Bovine
Package
100 μg ($399)
See all Histone H2A.X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 2-4 μg/ml
IHC-P 1:400
FACS 1μg per 10⁶ cells
ELISA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Product Note

The immunogen of this antibody is a synthetic peptide sequence surrounding phosphorylated Ser140. Some references call Ser140, by the name Ser139.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide sequence surrounding phosphorylated Ser140

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_625405

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80694

GTX80694 ICC/IF Image

Orthogonal

ICC/IF analysis of cells using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in Western Blot (WB). GTX80694

GTX80694 WB Image

Orthogonal

WB analysis of Jurkat cell untreated (Lane 1) and Jurkat cell stimulated with staurosporine (Lane 2) using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].
Dilution : 1 μg/ml

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in IHC (Paraffin sections) (IHC-P). GTX80694

GTX80694 IHC-P Image

IHC-P analysis of postnatal mouse (PN19) lung sections using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2]. Increased staining intensity was observed in a genetic mouse model with DNA damage in airway cells.
Antigen retrieval : heat induced epitope retrieval (HIER) method with sodium citrate buffer (pH 6.0)
Fixation : 4% Paraformaldehyde
Dilution : 1:400

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80694

GTX80694 ICC/IF Image

ICC/IF analysis of gamma irradiated HeLa cells using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [3F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80694

GTX80694 ICC/IF Image

Orthogonal

ICC/IF analysis of A549 cells treated with vehicle (control; 0.1% DMSO in media) or with 50 μM etoposide for 1 hour using GTX80694 Histone H2A.XS139ph (phospho Ser139) antibody [3F2].
Red : Primary antibody
Blue : Nuclei

REFERENCE

Application Reference

Wang YY et al. Cancer Chemother Pharmacol 2017; 79 (6) : 1129-1140 CYT-Rx20 inhibits ovarian cancer cells in vitro and in vivo through oxidative stress-induced DNA damage and cell apoptosis.
Application : WB
Reactivity : Human
Wang YY et al. Head Neck 2017; (Epub) Synthetic β-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction.
Application : WB
Reactivity : Human
Shang HS et al. Oncol Rep 2016; 36 (4) : 2207-15 Curcumin causes DNA damage and affects associated protein expression in HeLa human cervical cancer cells.
Smith S et al. PLoS One 2014; 9 (1) : e87203 Histone deacetylase inhibitors selectively target homology dependent DNA repair defective cells and elevate non-homologous endjoining activity.
Application : WB
Reactivity : Human
Lee JH et al. PLoS One 2014; 9 (6) : e97969 Direct Activation of ATM by Resveratrol under Oxidizing Conditions.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399