Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] (GTX628996) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] detects Histone H2A.XS139ph (phospho Ser139) protein at Nucleus by immunofluorescent analysis.
Sample: 10uM Cisplatin for 24 hrs HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] (GTX628996) diluted at 1:500.
Blue: Hoechst 33342 staining.