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Histone H2A.XS139ph (phospho Ser139) antibody [GT1021]

Orthogonal
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] used in Western Blot (WB). GTX628996
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628996

Cat. No. GTX628996

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1021

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Histone H2A.X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HCT116 (30 μM Cisplatin treatment for 24hr)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888084

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] used in Western Blot (WB). GTX628996

GTX628996 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] (GTX628996) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628996

GTX628996 ICC/IF Image

Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] detects Histone H2A.XS139ph (phospho Ser139) protein at Nucleus by immunofluorescent analysis.
Sample: 10uM Cisplatin for 24 hrs HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody [GT1021] (GTX628996) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Lin CC et al. Cell Death Differ 2020; 27 (7) : 2234-2247 RIPK3 upregulation confers robust proliferation and collateral cystine-dependence on breast cancer recurrence.
Chao-Chieh Lin et al. bioRxiv 2019; (Epub) RIPK3 upregulation confers robust proliferation and collateral cystine-dependence on breast cancer recurrence.
Application : ICC/IF
Reactivity : Mouse
Zhang P et al. Biochem Biophys Res Commun 2017; inhibition of b7-h3 reverses * resistance in human colorectal cancer cells.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations