Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 μg HCT116 with CPT 30 μM treatment 24 hr whole cell lysate/extract A. 30 μg HCT116 whole with CPT 30 uM treatment cell lysate/extract B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of histone H2A.X (phospho S139) protein by 2 μg histone H2A.X (phospho S139) antibody (GTX628789) 15% SDS-PAGE The immunoprecipitated histone H2A.X (phospho S139) protein was detected by Human histone H2A.X (phospho S139) antibody (GTX628789) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 μg) were separated by 12%-15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibody (GTX628789) diluted at 1:500. Blue: Hoechst 33342 staining.

Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 μM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis.
Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min.
Red: H2A.X protein stained by Histone H2A.X (phospho Ser139) antibody (GTX628789) diluted at 1:500.
Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract (untreated)
B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr)
15% SDS-PAGE
Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

The data was published in the journal Front Physiol in 2019. PMID: 31681015

The data was published in the journal PLoS Negl Trop Dis in 2017. PMID: 28783752

The data was published in the journal Sci Rep in 2020.PMID: 31980707

The data was published in the 2019 in Int J Mol Sci. PMID: 31717306

The data was published in the 2019 in Int J Mol Sci. PMID: 31717306