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Histone H2A.XS139ph (phospho Ser139) antibody [GT2311]

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Immunoprecipitation (IP). GTX628789
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in IHC (Paraffin sections) (IHC-P). GTX628789
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628789
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628789
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Western Blot (WB). GTX628789
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Western Blot (WB). GTX628789
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Western Blot (WB). GTX628789
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Western Blot (WB). GTX628789

Cat No. GTX628789

Host Mouse
Clonality Monoclonal
Clone Name GT2311
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HCT116 (30μM cisplatin treatment for 24hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , PC-12 (30uM cisplatin treatment for 24h) , U2OS treated UV (50 J/m2)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H2A.XS139ph (phospho Ser139). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H2A histone family member X,H2A.X,H2A/X,H2AX

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Immunoprecipitation (IP). GTX628789

GTX628789 IP Image

Histone H2A.X (phospho S139) antibody immunoprecipitates histone H2A.X (phospho S139) protein in IP experiments. IP Sample: 500 μg HCT116 with CPT 30 μM treatment 24 hr whole cell lysate/extract A. 30 μg HCT116 whole with CPT 30 uM treatment cell lysate/extract B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of histone H2A.X (phospho S139) protein by 2 μg histone H2A.X (phospho S139) antibody (GTX628789) 15% SDS-PAGE The immunoprecipitated histone H2A.X (phospho S139) protein was detected by Human histone H2A.X (phospho S139) antibody (GTX628789) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in IHC (Paraffin sections) (IHC-P). GTX628789

GTX628789 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] (GTX628789) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628789

GTX628789 ICC/IF Image

Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis.
Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min.
Red: H2A.X protein stained by Histone H2A.X (phospho Ser139) antibody (GTX628789) diluted at 1:500.
Green: alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628789

GTX628789 ICC/IF Image

Histone H2A.X antibody detects H2AFX protein at nuclear by immunofluorescent analysis. Sample: 10μM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.Xantibody (GTX628789) diluted at 1:500. Blue: Hoechst 33342 staining.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Western Blot (WB). GTX628789

GTX628789 WB Image

Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract (untreated)
B. 30 µg NIH-3T3 whole cell lysate/extract (30µM cisplatin treatment for 24hr)
15% SDS-PAGE
Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Western Blot (WB). GTX628789

GTX628789 WB Image

Histone H2A.X (phospho S139) antibody [GT2311] detects Histone H2A.X (phospho S139) [GT2311] protein by western blot analysis. Un-treated (-) and treated (+, 30 µM Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Western Blot (WB). GTX628789

GTX628789 WB Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 50 J/m2 UV treatment) U2OS whole cell extracts (16 µg) were separated by 12%-15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX628789) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [GT2311] used in Western Blot (WB). GTX628789

GTX628789 WB Image

Histone H2A.X (phospho S139) antibody [GT2311] detects H2AFX protein by western blot analysis.
A. 30 µg HCT116 whole cell lysate/extract (untreated)
B. 30 µg HCT116 whole cell lysate/extract (30 µM cisplatin treatment for 24hr)
12% SDS-PAGE
Histone H2A.X (phospho S139) antibody [GT2311] (GTX628789) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169