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Histone H2A.XS139ph (phospho Ser139) antibody [RM224]

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [RM224] used in Western Blot (WB). GTX33597
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [RM224] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33597

Cat No. GTX33597

Host Rabbit
Clonality Monoclonal
Clone Name RM224
Isotype IgG
Application WB, ICC/IF, ELISA, Multiplexing
Reactivity Human
APPLICATION

Application Note

Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 2 ug/mL, Immunohistochemistry (IHC): 0.5 – 5 ug/mL, Immunocytochemistry (ICC): 0.5 – 6 ug/mL.

Calculated MW

15 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139)

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H2A Histone Family Member X,H2A.X,H2A/X,H2Ax,H2Afx

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Histone H2A.XS139ph (phospho Ser139) antibody [RM224] used in Western Blot (WB). GTX33597

GTX33597 WB Image

Western Blot of acid extracts of HeLa cells treatedor non-treated with UV. Using RM224 at 0.5 ug/mL, showed a band of Histone H2A.X phosphorylatedat serine 139 in HeLa cells.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody [RM224] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33597

GTX33597 ICC/IF Image

Immunocytochemistry of HeLa cells using gammaH2AX Rabbit mAb RM224 (red). Actin filamentshave been labeled with fluorescein phalloidin (green).

Package List Price ($)
$ 349