Histone H2A.XS139ph (phospho Ser139) antibody [RM224]

Cat. No. GTX33597

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM224
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human
Package	100 μg ($449)

See all Histone H2A.X products

Datasheet

View product citations for antibody GTX33597 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 μg/mL - 2 μg/mL
ICC/IF	0.5 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Product Note

This antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,

Cellular Localization

Nucleus,Chromosome

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

Database

Gene ID: 3014 H2AX
UniProt: P16104 H2AX

Research Area

DATA IMAGES

GTX33597 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33597 Histone H2A.XS139ph (phospho Ser139) antibody [RM224].
Red : Primary antibody
Green : Actin

GTX33597 WB Image

WB analysis of acid extracts of HeLa cells treatedor non-treated with UV using GTX33597 Histone H2A.XS139ph (phospho Ser139) antibody [RM224].
Dilution : 0.5μg/ml

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty