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Histone H2A.XS139ph (phospho Ser139) antibody

GTX127340_42851_20170518_WB_cisplatin_18121410_699.jpg
GTX127340_40982_WB_M_cisplatin_18121410_740.jpg
GTX127340_40982_WB_UV_18121410_981.jpg
GTX127340_40982_IFA_18121410_530.jpg
GTX127340_41008_IFA_18121410_774.jpg
GTX127340_40982_20141128_IHC_18121410_954.jpg
GTX127340_40982_20150903_WB_cisplatin_18121410_304.jpg
GTX127340_43467_20190201_WB_treatment_Cisplatin_19030801_820.jpg

Cat No. GTX127340

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Wm
Reactivity Human, Mouse, Rat, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HCT116 , HCT116 (30 μM cisplatin treatment for 24 hr) , HCT116 (UVB treatment 50mJ for 2hr) , HCT116 (UVB treatment 50mJ for 6hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , PC-12 (30uM cisplatin treatment for 24hr)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Histone H2A.XS139ph (phospho Ser139). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H2A Histone Family Member X,H2A.X,H2A/X,H2Ax,H2Afx

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX127340_42851_20170518_WB_cisplatin_18121410_699.jpg

GTX127340 WB Image

Untreated (–) and treated (+) HCT-116 whole cell extract (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127340_40982_WB_M_cisplatin_18121410_740.jpg

GTX127340 WB Image

Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract (untreated)
B. 30 µg NIH-3T3 whole cell lysate/extract (30µM cisplatin treatment for 24hr)
15% SDS-PAGE
Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127340_40982_WB_UV_18121410_981.jpg

GTX127340 WB Image

Histone H2A.X (phospho Ser139) antibody detects H2AFX protein by western blot analysis.
A. 30 µg HCT116 whole cell lysate/extract (untreated for 2hr)
B. 30 µg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 2hr)
C. 30 µg HCT116 whole cell lysate/extract (untreated for 6hr)
D. 30 µg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 6hr)
15% SDS-PAGE
Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127340_40982_IFA_18121410_530.jpg

GTX127340 ICC/IF Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX127340_41008_IFA_18121410_774.jpg

GTX127340 ICC/IF Image

Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis. Sample: 10uM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.X (phospho Ser139) antibody (GTX127340) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

GTX127340_40982_20141128_IHC_18121410_954.jpg

GTX127340 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX127340_40982_20150903_WB_cisplatin_18121410_304.jpg

GTX127340 WB Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 30 µM cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127340_43467_20190201_WB_treatment_Cisplatin_19030801_820.jpg

GTX127340 WB Image

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Histone H2A.XS139ph (phospho Ser139) antibody Cat No. GTX127340

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Sep-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : M10 cells
Amount used : 30μg
Blocking : 0.05% not fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169