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Histone H2A.XS139ph (phospho Ser139) antibody

Orthogonal
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340

Cat. No. GTX127340

Reference 40
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Histone H2A.X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

HCT116 (UVB treatment 50mJ for 2hr) , HCT116 (UVB treatment 50mJ for 6hr) , NIH-3T3 (30μM cisplatin treatment for 24hr) , HCT-116 (30 uM cisplatin treatment for 24 hr)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of human Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885642

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

,H2A histone family member X,gamma H2A.X,gamma H2AX,H2A.X,H2A/X,H2AX,H2AFX,

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340

GTX127340 WB Image

Orthogonal

Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract (untreated)
B. 30 μg NIH-3T3 whole cell lysate/extract (30μM cisplatin treatment for 24hr)
15% SDS-PAGE
Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340

GTX127340 WB Image

Orthogonal

Histone H2A.X (phospho Ser139) antibody detects H2AFX protein by western blot analysis.
A. 30 μg HCT116 whole cell lysate/extract (untreated for 2hr)
B. 30 μg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 2hr)
C. 30 μg HCT116 whole cell lysate/extract (untreated for 6hr)
D. 30 μg HCT116 whole cell lysate/extract (UVB treatment 50mJ for 6hr)
15% SDS-PAGE
Histone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127340

GTX127340 ICC/IF Image

Orthogonal

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340

GTX127340 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127340

GTX127340 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340

GTX127340 WB Image

Orthogonal

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340

GTX127340 WB Image

Orthogonal

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127340

GTX127340 ICC/IF Image

Orthogonal

The data was published in the 2022 in Cell Death Dis. PMID: 35688824

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127340

GTX127340 ICC/IF Image

Orthogonal

The data was published in the journal FEBS Open Bio in 2018. PMID: 29928579

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340

GTX127340 WB Image

The data was published in the journal Cell Death Discov in 2016. PMID: 27551500

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340

GTX127340 WB Image

The data was published in the journal Cell Death Discov in 2016. PMID: 27551500

REFERENCE

Application Reference

LH Giang et al. J Exp Clin Cancer Res 2023; 42 (1) : 346 Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor
Application : WB
Reactivity : Human
Jeong H et al. Cells 2022; 11 (24) TRIP13 Participates in Immediate-Early Sensing of DNA Strand Breaks and ATM Signaling Amplification through MRE11.
Zhou X et al. Transl Cancer Res 2022; 11 (8) : 2767-2782 MicroRNA expression profiles in extracellular vesicles and intracellular of AURKA inhibitor-induced senescent neuroblastoma cells.
Application : ICC/IF
Reactivity : Human
Hsueh-Tzu Shih et al. Cell Death Dis 2022; 13 (6) : 546 DNMT3b protects centromere integrity by restricting R-loop-mediated DNA damage.
Application : WB
Reactivity : Human
Tung Chao et al. Autophagy 2021; 17 (11) : 3444-3460 Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability.
Application : WB, IHC-P
Reactivity : Human, Mouse
Show More +
Submit a Reference

REVIEW

Histone H2A.XS139ph (phospho Ser139) antibody Cat. No. GTX127340

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340
Date : Anonymous submitted on 23-Jun-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20 μg
Blocking : 5% non-fat milk in TBST, 22°C, 0.5Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127340
Date : Anonymous submitted on 15-Sep-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : M10 cells
Amount used : 30μg
Blocking : 0.05% not fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
LH Giang et al. J Exp Clin Cancer Res 2023; 42 (1):346 Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor
Application : WB
Reactivity : Human
Jeong H et al. Cells 2022; 11 (24) TRIP13 Participates in Immediate-Early Sensing of DNA Strand Breaks and ATM Signaling Amplification through MRE11.
Zhou X et al. Transl Cancer Res 2022; 11 (8):2767-2782 MicroRNA expression profiles in extracellular vesicles and intracellular of AURKA inhibitor-induced senescent neuroblastoma cells.
Application : ICC/IF
Reactivity : Human
Hsueh-Tzu Shih et al. Cell Death Dis 2022; 13 (6):546 DNMT3b protects centromere integrity by restricting R-loop-mediated DNA damage.
Application : WB
Reactivity : Human
Tung Chao et al. Autophagy 2021; 17 (11):3444-3460 Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability.
Application : WB, IHC-P
Reactivity : Human, Mouse
Vanhunsel S et al. NPJ Aging Mech Dis 2021; 7 (1):22 The killifish visual system as an in vivo model to study brain aging and rejuvenation.
Application : IHC-Fr
Yang JL et al. In Vivo 2021; 35 (5):2687-2696 Ouabain Induces DNA Damage in Human Osteosarcoma U-2 OS Cells and Alters the Expression of DNA Damage and DNA Repair-associated Proteins.
Application : WB
Zhao T et al. Cell Death Dis 2021; 12 (2):151 Loss-of-function of p53 isoform Δ113p53 accelerates brain aging in zebrafish.
Application : IHC-Fr
Reactivity : Zebrafish
Cheng YC et al. In Vivo 2021; 35 (2):833-843 Pre-Treatment of Pterostilbene Enhances H2O2-induced Cell Apoptosis Through Caspase-dependent Pathway in Human Keratinocyte Cells.
Application : WB
Reactivity : Human
Ye S et al. Cell Death Dis 2020; 11 (7):568 p53 isoform Δ113p53 promotes zebrafish heart regeneration by maintaining redox homeostasis.
Lu KW et al. Anticancer Res 2020; 40 (12):6869-6877 Maslinic Acid Induces DNA Damage and Impairs DNA Repair in Human Cervical Cancer HeLa Cells.
Reactivity : Human
Wang YY et al. ACS Omega 2020; 5 (12):7018-7024 Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo.
Application : WB
Reactivity : Human
Wang YY et al. Biochem Pharmacol 2020; 171:113713 Orabase-formulated gentian violet effectively improved oral potentially malignant disorder in vitro and in vivo.
Application : WB
Reactivity : Human
Shenzhen Huang et al. RSC Adv 2019; 9 (16):9323-9330 Discovery of human TyrRS inhibitors by structure-based virtual screening, structural optimization, and bioassays.
Application : WB
Reactivity : Human
van Gastel J et al. Aging (Albany NY) 2019; 11 (23):11268-11313 The RXFP3 receptor is functionally associated with cellular responses to oxidative stress and DNA damage.
Application : WB
Reactivity : Human
Gizak A et al. Cell Death Dis 2019; 10 (10):712 Targeting a moonlighting function of aldolase induces apoptosis in cancer cells.
Application : ICC/IF
Reactivity : Mouse
Novoa B et al. Aging Cell 2019;:e13020 Rag1 immunodeficiency-induced early aging and senescence in zebrafish are dependent on chronic inflammation and oxidative stress.
Application : ICC/IF
Reactivity : Zebrafish
Lan Y et al. Br J Dermatol 2019; OPN3 is a key regulator of UVA-induced photoaging in human dermal fibroblast cells.
Application : WB, ICC/IF
Reactivity : Human
Li CH et al. Toxins (Basel) 2019; 11 (5) Recombinant Aflatoxin-Degrading F420H2-Dependent Reductase from Mycobacterium smegmatis Protects Mammalian Cells from Aflatoxin Toxicity.
Application : ICC/IF
Shenzhen Huang et al. RSC Adv. 2019; 9:9323-9330 Discovery of human TyrRS inhibitors by structure-based virtual screening, structural optimization, and bioassays.
Application : WB
Reactivity : Human
Wang YY et al. Oral Dis 2019; 25 (3):758-771 MAL-PDT inhibits oral precancerous cells and lesions via autophagic cell death.
Application : WB
Reactivity : Human
Huang AC et al. Anticancer Res 2019; 39 (4):1839-1847 Casticin Induces DNA Damage and Impairs DNA Repair in Human Bladder Cancer TSGH-8301 Cells.
Application : WB
Reactivity : Human
Sakamoto A et al. Cancer Sci 2019; 110 (1):269-278 Pyruvate secreted from patient-derived cancer-associated fibroblasts supports survival of primary lymphoma cells.
Application : WB
Reactivity : Human
Oku Y et al. FEBS Open Bio 2018; 8 (6):1001-1012 Augmentation of the therapeutic efficacy of WEE1 kinase inhibitor AZD1775 by inhibiting the YAP-E2F1-DNA damage response pathway axis.
Tu HJ et al. Clin Epigenetics 2018; 10 (1):162 The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells.
Application : WB
Reactivity : Human
Chen CC et al. Cell Death Dis 2018; 9 (5):543 Corylin increases the sensitivity of hepatocellular carcinoma cells to chemotherapy through long noncoding RNA RAD51-AS1-mediated inhibition of DNA repair.
Application : ICC/IF
Reactivity : Human
Ratnadiwakara M et al. Elife 2018; 7 SRSF3 promotes pluripotency through Nanog mRNA export and coordination of the pluripotency gene expression program.
Application : WB
Reactivity : Mouse
Oku Y. et al. FEBS openbio 2018; (Epub) Augmentation of the therapeutic efficacy of WEE1 kinase inhibitor AZD1775 by inhibiting the YAP–E2F1–DNA damage response pathway axis.
Application : ICC/IF
Reactivity : Human
Ma N et al. bioRxiv 2018; (Epub) Label-free assessment of pre-implantation embryo quality by the Fluorescence Lifetime Imaging Microscopy (FLIM)-phasor approach.
Application : IHC-Wm
Reactivity : Mouse
Calo E et al. Nature 2018; 554 (7690):112-117 Tissue-selective effects of nucleolar stress and rDNA damage in developmental disorders.
Application : IHC-Wm
Reactivity : Zebrafish
Koo GB et al. Sci Rep 2017; 7 (1):3332 Nuclear TRADD prevents DNA damage-mediated death by facilitating non-homologous end-joining repair.
Application : WB, ICC/IF
Reactivity : Mouse
Chien JY et al. Cell Death Discov 2016; 2:16006 αTAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells.
Application : WB
Reactivity : Human
Tsai CH et al. Life Sci 2016; (Epub) 3'-Hydroxy-4'-methoxy-β-methyl-β-nitrostyrene inhibits tumor growth through ROS generation and GSH depletion in lung cancer cells.
Application : WB
Reactivity : Human
Wei RJ et al. Toxicol Appl Pharmacol 2016; (Epub) A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells.
Application : WB
Reactivity : Human
Liu MK et al. Int J Mol Sci 2016; 17 (6) Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response.
Application : WB
Reactivity : Human
Tsao N et al. Biochem J 2016; 473 (9):1237-45 The direct interaction of NME3 with Tip60 in DNA repair.
Application : ICC/IF
Reactivity : Human
Yao-Yu H et al. Oncotarget 2016; (Epub) Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.
Application : WB
Reactivity : Human
Tsao N et al. Cell Cycle 2015; 14 (3):354-63 The contribution of CMP kinase to the efficiency of DNA repair.
Application : ICC/IF
Reactivity : Human
Percival SM et al. Dis Model Mech 2015; 8 (8):941-55 Variations in dysfunction of sister chromatid cohesion in esco2 mutant zebrafish reflect the phenotypic diversity of Roberts syndrome.
Application : WB
Reactivity : Zebrafish
May EW et al. Pharmacol Res 2014; (Epub) identification of up- and down-regulated proteins in *-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of *-associated resistance.
Application : WB
Reactivity : Human
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