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Histone H2A.XS139ph (phospho Ser139) antibody

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342

Cat. No. GTX127342

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:2000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC 1:100-1:2000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of zebrafish Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2833105

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H2A histone family, member X , zgc:56329

Cellular Localization

Nucleus

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Database

Research Area

DATA IMAGES

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) dilution: 1:100.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342

GTX127342 IHC Image

H2afx antibody detects H2afx protein at nucleus on UV-irradiated whole-mount zebrafish embryos (under panel) by immunohistochemical analysis.
Sample: H2afx antibody (GTX127342) dilution: 1:2000.
Image provided with permission courtesy of Dr. James Amatruda.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342

GTX127342 WB Image

Untreated (–) and treated (+) Zebrafish embryos extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on UV-irradiated whole mount zebrafish (right panel) by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 days-post-fertilization zebrafish embryo.
Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342

GTX127342 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded human breast carcinoma.
Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Histone H2A.XS139ph (phospho Ser139) antibody Cat. No. GTX127342

Rating ( Average 5 based on 4 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 2 users reviews)
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2018.PMID: 29419415
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2018.PMID: 29419415
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31882844
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 30837464
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169