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Histone H2A.XS139ph (phospho Ser139) antibody

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342

Cat. No. GTX127342

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:2000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC 1:100-1:2000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of zebrafish Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2833105

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family, member X , zgc:56329

Cellular Localization

Nucleus

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Database

Research Area

DATA IMAGES

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) dilution: 1:100.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on UV-irradiated whole mount zebrafish (right panel) by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 days-post-fertilization zebrafish embryo.
Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342

GTX127342 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded human breast carcinoma.
Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342

GTX127342 IHC Image

H2afx antibody detects H2afx protein at nucleus on UV-irradiated whole-mount zebrafish embryos (under panel) by immunohistochemical analysis.
Sample: H2afx antibody (GTX127342) dilution: 1:2000.
Image provided with permission courtesy of Dr. James Amatruda.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342

GTX127342 WB Image

Untreated (–) and treated (+) Zebrafish embryos extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342

GTX127342 ICC/IF Image

The data was published in the journal Dis Model Mech in 2018.PMID: 29419415

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

The data was published in the journal Sci Rep in 2019. PMID: 31882844

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

The data was published in the journal Nat Commun in 2019. PMID: 30837464

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342

GTX127342 ICC/IF Image

The data was published in the journal Dis Model Mech in 2018.PMID: 29419415

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169