GeneTex
United States (US)

Histone H2A.XS139ph (phospho Ser139) antibody

Orthogonal
GTX127342_41906_20141201_IHC-Wm_Z_18121410_627.jpg
GTX127342_42179_20160509_IHC-Wm_Z_18121410_920.jpg
GTX127342_42389_20160204_WB_Z_UVC_18121410_915.jpg
GTX127342_40996_IHC_18121410_709.jpg

Cat No. GTX127342

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:2000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC 1:100-1:2000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

3dpf zebrafish embryos , 3dpf zebrafish embryos (UVC-treated 8000J/m2)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66mg/ml

Antigen Species

Zebrafish

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around zebrafish Histone H2A.XS139ph (phospho Ser139). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

gamma H2AX, H2A.XS139ph,Phospho Histone H2A.X (pS139 )

Cellular Localization

Nucleus

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
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Database

Research Area

DATA IMAGES
GTX127342_41906_20141201_IHC-Wm_Z_18121410_627.jpg

GTX127342 IHC-Wm Image

Orthogonal

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on UV-irradiated zebrafish embryos (right panel) by whole mount immunohistochemical analysis.
Samples: 24 hours-post-fertilization zebrafish embryo.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) dilution: 1:100.

GTX127342_42179_20160509_IHC-Wm_Z_18121410_920.jpg

GTX127342 IHC-Wm Image

Orthogonal

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) dilution: 1:100.

GTX127342_42389_20160204_WB_Z_UVC_18121410_915.jpg

GTX127342 WB Image

Orthogonal

Untreated (–) and treated (+) zebrafish embryos extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000.

GTX127342_40996_IHC_18121410_709.jpg

GTX127342 IHC Image

Orthogonal

H2afx antibody detects H2afx protein at nucleus on UV-irradiated whole-mount zebrafish embryos (under panel) by immunohistochemical analysis.
Sample: H2afx antibody (GTX127342) dilution: 1:2000.
Image provided with permission courtesy of Dr. James Amatruda.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199