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Histone H2A.XS139ph (phospho Ser139) antibody

Orthogonal
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

Cat. No. GTX127342

Reference 44
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Histone H2A.X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:2000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC 1:100-1:2000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of zebrafish Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2833105

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family, member X , zgc:56329

Cellular Localization

Nucleus

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Database

Research Area

DATA IMAGES

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Orthogonal

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) dilution: 1:100.
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342

GTX127342 WB Image

Orthogonal

Untreated (–) and treated (+) Zebrafish embryos extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342

GTX127342 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded human breast carcinoma.
Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342

GTX127342 IHC Image

Orthogonal

H2afx antibody detects H2afx protein at nucleus on UV-irradiated whole-mount zebrafish embryos (under panel) by immunohistochemical analysis.
Sample: H2afx antibody (GTX127342) dilution: 1:2000.
Image provided with permission courtesy of Dr. James Amatruda.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Orthogonal

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on UV-irradiated whole mount zebrafish (right panel) by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 days-post-fertilization zebrafish embryo.
Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

Application Reference

P Cacialli et al. Stem Cell Reports 2023; 18 (7) : 1534-1546 Minichromosome maintenance protein 10 (mcm10) regulates hematopoietic stem cell emergence in the zebrafish embryo
Application : WB, ICC/IF
M El Ma? et al. Nat Aging 2023; Gut-specific telomerase expression counteracts systemic aging in telomerase-deficient zebrafish
Application : IHC-Fr
Reactivity : Zebrafish
Song J et al. NPJ Regen Med 2023; 8 (1) : 20 The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway.
Application : IHC-Wm
Reactivity : Zebrafish
Babu S et al. Elife 2022; 11 Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina.
Application : IHC-P, IHC-Fr
Reactivity : Zebrafish
Ellis PS et al. Immun Ageing 2022; 19 (1) : 31 A subset of gut leukocytes has telomerase-dependent "hyper-long" telomeres and require telomerase for function in zebrafish.
Show More +
Submit a Reference

REVIEW

Histone H2A.XS139ph (phospho Ser139) antibody Cat. No. GTX127342

Rating
( Average 5 based on 4 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 2 users reviews)
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2018.PMID: 29419415
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2018.PMID: 29419415
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31882844
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 30837464
Submit a Review
Application Reference
P Cacialli et al. Stem Cell Reports 2023; 18 (7):1534-1546 Minichromosome maintenance protein 10 (mcm10) regulates hematopoietic stem cell emergence in the zebrafish embryo
Application : WB, ICC/IF
M El Ma? et al. Nat Aging 2023; Gut-specific telomerase expression counteracts systemic aging in telomerase-deficient zebrafish
Application : IHC-Fr
Reactivity : Zebrafish
Song J et al. NPJ Regen Med 2023; 8 (1):20 The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathway.
Application : IHC-Wm
Reactivity : Zebrafish
Tian Y et al. Nat Commun 2022; 13 (1):5456 WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome.
Ma J et al. iScience 2022; 25 (9):104957 Ercc2/Xpd deficiency results in failure of digestive organ growth in zebrafish with elevated nucleolar stress.
Xie H et al. J Mol Cell Biol 2022; 14 (7) Cilia regulate meiotic recombination in zebrafish.
Application : IHC-Fr
Reactivity : Zebrafish
Babu S et al. Elife 2022; 11 Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina.
Application : IHC-P, IHC-Fr
Reactivity : Zebrafish
Ellis PS et al. Immun Ageing 2022; 19 (1):31 A subset of gut leukocytes has telomerase-dependent "hyper-long" telomeres and require telomerase for function in zebrafish.
Jason K H Lai et al. Elife 2022; 11 DNA-damage induced cell death in yap1;wwtr1 mutant epidermal basal cells.
Application : WB, IHC-Wm
Reactivity : Zebrafish
Tam?s Annus et al. Cell Death Dis 2022; 13 (4):363 Bloom syndrome helicase contributes to germ line development and longevity in zebrafish.
Reactivity : Zebrafish
Ilaria Dutto et al. Elife 2022; 11 Pathway-specific effects of ADSL deficiency on neurodevelopment.
Application : ICC/IF
Reactivity : Human
Vanoevelen JM et al. Acta Neuropathol 2022; 143 (2):245-262 DTYMK is essential for genome integrity and neuronal survival.
Zada D et al. Mol Cell 2021; 81 (24):4979-4993.e7 Parp1 promotes sleep, which enhances DNA repair in neurons.
Ringaile Zaksauskaite et al. Sci Adv 2021; 7 (5) Tdp1 protects from topoisomerase 1-mediated chromosomal breaks in adult zebrafish but is dispensable during larval development.
Application : WB
Reactivity : Zebrafish
Mart?nez-Morcillo FJ et al. PLoS Biol 2021; 19 (11):e3001455 NAMPT-derived NAD+ fuels PARP1 to promote skin inflammation through parthanatos cell death.
Application : IHC-Wm
Reactivity : Zebrafish
Riemslagh FW et al. Dis Model Mech 2021; (Epub) Reduction of oxidative stress suppresses poly-GR mediated toxicity in zebrafish embryos.
Application : IHC-P
Reactivity : Zebrafish
Vanhunsel S et al. NPJ Aging Mech Dis 2021; 7 (1):22 The killifish visual system as an in vivo model to study brain aging and rejuvenation.
Application : IHC-Fr
Annus T. et al. bioRxiv 2021; (Epub) Bloom syndrome helicase contributes to germ line development and longevity in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Mohd Noor Hidayat Adenan et al. Molecules 2021; 26 (6):1557 Radioprotective Effects of Kelulut Honey in Zebrafish Model.
Application : IHC-Wm
Reactivity : Zebrafish
Kim HR et al. Oncotarget 2020; 11 (13):1109-1130 Investigation of the role of VHL-HIF signaling in DNA repair and apoptosis in zebrafish.
Giunco S et al. Cancers (Basel) 2020; 12 (8) Anti-Proliferative and Pro-Apoptotic Effects of Short-Term Inhibition of Telomerase In Vivo and in Human Malignant B Cells Xenografted in Zebrafish.
Application : WB
Reactivity : Zebrafish
El Ma? M et al. Elife 2020; 9 Opposing p53 and mTOR/AKT promote an in vivo switch from apoptosis to senescence upon telomere shortening in zebrafish.
Application : WB
Reactivity : Zebrafish
Ren F et al. Toxicol Appl Pharmacol 2020; 398:115029 Resveratrol protects against PM2.5-induced heart defects in zebrafish embryos as an antioxidant rather than as an AHR antagonist.
Application : IHC-Wm
Reactivity : Zebrafish
Xie H et al. PLoS Genet 2020; 16 (3):e1008655 E2f5 is a versatile transcriptional activator required for spermatogenesis and multiciliated cell differentiation in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Oh CK et al. Hematol Oncol 2020; (Epub) FAM213A is linked to prognostic significance in acute myeloid leukemia through regulation of oxidative stress and myelopoiesis.
Application : IHC-Wm
Reactivity : Zebrafish
Adriana Petrovici et al. J. Mar. Sci. Eng. 2020; 8 (2):73 Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers.
Application : IHC-P
Reactivity : Zebrafish
Murat El Houdigui S et al. Sci Rep 2019; 9 (1):20241 A systems biology approach reveals neuronal and muscle developmental defects after chronic exposure to ionising radiation in zebrafish.
Honjo Y et al. Cell Biol Int 2019; 43 (5):516-527 Cellular responses to ionizing radiation change quickly over time during early development in zebrafish.
Zada D et al. Nat Commun 2019; 10 (1):895 Sleep increases chromosome dynamics to enable reduction of accumulating DNA damage in single neurons.
Li G et al. Front Mol Neurosci 2019; 12:126 Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Zebrafish Development.
Singh AN et al. EMBO J 2019; 38 (21):e102361 The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.
HR Kim et al. bioRxiv 2019; (Epub) The investigation of the role of VHL-HIF signaling in DNA repair and apoptosis in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
He J et al. Hepatology 2019; (Epub) mTORC1 Signaling is Required for the Dedifferentiation from Biliary Cell to Bi-potential Progenitor Cell in Zebrafish Liver Regeneration.
Application : IHC-Wm
Reactivity : Zebrafish
Laura E. Schultz-Rogers et al. bioRxiv 2018; (Epub) Retinoblastoma binding protein 4 maintains cycling neural stem cells and prevents DNA damage and Tp53-dependent apoptosis in rb1 mutant neural progenitors.
Application : IHC-Wm
Reactivity : Zebrafish
ZekunWang et al. BBRC 2018; 505 (2):378-384 Loss of Leucyl-tRNA synthetase b leads to ILFS1-like symptoms in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Gonzalez-Rosa JM et al. Dev Cell 2018; 44 (4):433-446.e7 Myocardial Polyploidization Creates a Barrier to Heart Regeneration in Zebrafish.
Application : IHC-P
Reactivity : Zebrafish
Sorrells S et al. Dis Model Mech 2018; (Epub) Spliceosomal components protect embryonic neurons from R-loop-mediated DNA damage and apoptosis.
Application : ICC/IF
Reactivity : Zebrafish
Kroeger PT Jr et al. Dev Biol 2017; (Epub) The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development.
Application : IHC-Wm
Cao X et al. Proc Natl Acad Sci U S A 2017; 114 (4):687-692 Acetylation promotes TyrRS nuclear translocation to prevent oxidative damage.
Application : IHC-Wm
Reactivity : Zebrafish
Chun C et al. Cell Cycle 2016; 15 (7):957-62 The homologous recombination component EEPD1 is required for genome stability in response to developmental stress of vertebrate embryogenesis.
Application : IHC-Fr
Reactivity : Zebrafish
Zhou R et al. Mutat Res 2016; 793-794:41-50 Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos.
Application : WB
Reactivity : Zebrafish
Carneiro MC et al. PLoS Genet 2016; 12 (1):e1005798 Short Telomeres in Key Tissues Initiate Local and Systemic Aging in Zebrafish.
Application : IHC-P
Reactivity : Zebrafish
Bednarek D et al. Cell Rep 2015; 12 (10):1691-703 Telomerase Is Essential for Zebrafish Heart Regeneration.
Application : WB, IHC-P
Reactivity : Zebrafish
Sasaki T et al. PLoS Genet 2014; 10 (6):e1004409 Aberrant Autolysosomal Regulation Is Linked to The Induction of Embryonic Senescence: Differential Roles of Beclin 1 and p53 in Vertebrate Spns1 Deficiency.
Application : IHC-Wm
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169