GeneTex

  • Country / Location Selection

United States (US)

Histone H2A.XS139ph (phospho Ser139) antibody

Orthogonal
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342

Cat. No. GTX127342

Reference 35
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Histone H2A.X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:2000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC 1:100-1:2000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of zebrafish Histone H2A.X. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2833105

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H2A histone family, member X , zgc:56329

Cellular Localization

Nucleus

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Database

Research Area

DATA IMAGES

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Orthogonal

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) dilution: 1:100.
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunohistochemistry (IHC). GTX127342

GTX127342 IHC Image

Orthogonal

H2afx antibody detects H2afx protein at nucleus on UV-irradiated whole-mount zebrafish embryos (under panel) by immunohistochemical analysis.
Sample: H2afx antibody (GTX127342) dilution: 1:2000.
Image provided with permission courtesy of Dr. James Amatruda.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Western Blot (WB). GTX127342

GTX127342 WB Image

Orthogonal

Untreated (–) and treated (+) Zebrafish embryos extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342

GTX127342 IHC-Wm Image

Orthogonal

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on UV-irradiated whole mount zebrafish (right panel) by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 days-post-fertilization zebrafish embryo.
Green: Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Paraffin sections) (IHC-P). GTX127342

GTX127342 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded human breast carcinoma.
Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Jason K H Lai et al. Elife 2022; 11 DNA-damage induced cell death in yap1;wwtr1 mutant epidermal basal cells.
Application : WB, IHC-Wm
Reactivity : Zebrafish
Tam?s Annus et al. Cell Death Dis 2022; 13 (4) : 363 Bloom syndrome helicase contributes to germ line development and longevity in zebrafish.
Reactivity : Zebrafish
Ilaria Dutto et al. Elife 2022; 11 Pathway-specific effects of ADSL deficiency on neurodevelopment.
Application : ICC/IF
Reactivity : Human
Vanoevelen JM et al. Acta Neuropathol 2022; 143 (2) : 245-262 DTYMK is essential for genome integrity and neuronal survival.
Ringaile Zaksauskaite et al. Sci Adv 2021; 7 (5) Tdp1 protects from topoisomerase 1-mediated chromosomal breaks in adult zebrafish but is dispensable during larval development.
Application : WB
Reactivity : Zebrafish
Show More +
Submit a Reference

REVIEW

Histone H2A.XS139ph (phospho Ser139) antibody Cat. No. GTX127342

Rating
( Average 5 based on 4 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 2 users reviews)
IHC (Whole mount)(IHC-Wm)
( Average 5 based on 2 users reviews)
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2018.PMID: 29419415
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127342
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Dis Model Mech in 2018.PMID: 29419415
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31882844
Anti-Histone H2A.XS139ph (phospho Ser139) antibody used in IHC (Whole mount) (IHC-Wm). GTX127342
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 30837464
Submit a Review
Application Reference
Jason K H Lai et al. Elife 2022; 11 DNA-damage induced cell death in yap1;wwtr1 mutant epidermal basal cells.
Application : WB, IHC-Wm
Reactivity : Zebrafish
Tam?s Annus et al. Cell Death Dis 2022; 13 (4):363 Bloom syndrome helicase contributes to germ line development and longevity in zebrafish.
Reactivity : Zebrafish
Ilaria Dutto et al. Elife 2022; 11 Pathway-specific effects of ADSL deficiency on neurodevelopment.
Application : ICC/IF
Reactivity : Human
Vanoevelen JM et al. Acta Neuropathol 2022; 143 (2):245-262 DTYMK is essential for genome integrity and neuronal survival.
Ringaile Zaksauskaite et al. Sci Adv 2021; 7 (5) Tdp1 protects from topoisomerase 1-mediated chromosomal breaks in adult zebrafish but is dispensable during larval development.
Application : WB
Reactivity : Zebrafish
Mart?nez-Morcillo FJ et al. PLoS Biol 2021; 19 (11):e3001455 NAMPT-derived NAD+ fuels PARP1 to promote skin inflammation through parthanatos cell death.
Application : IHC-Wm
Reactivity : Zebrafish
Riemslagh FW et al. Dis Model Mech 2021; (Epub) Reduction of oxidative stress suppresses poly-GR mediated toxicity in zebrafish embryos.
Application : IHC-P
Reactivity : Zebrafish
Vanhunsel S et al. NPJ Aging Mech Dis 2021; 7 (1):22 The killifish visual system as an in vivo model to study brain aging and rejuvenation.
Application : IHC-Fr
Annus T. et al. bioRxiv 2021; (Epub) Bloom syndrome helicase contributes to germ line development and longevity in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Mohd Noor Hidayat Adenan et al. Molecules 2021; 26 (6):1557 Radioprotective Effects of Kelulut Honey in Zebrafish Model.
Application : IHC-Wm
Reactivity : Zebrafish
Kim HR et al. Oncotarget 2020; 11 (13):1109-1130 Investigation of the role of VHL-HIF signaling in DNA repair and apoptosis in zebrafish.
Giunco S et al. Cancers (Basel) 2020; 12 (8) Anti-Proliferative and Pro-Apoptotic Effects of Short-Term Inhibition of Telomerase In Vivo and in Human Malignant B Cells Xenografted in Zebrafish.
Application : WB
Reactivity : Zebrafish
El Ma? M et al. Elife 2020; 9 Opposing p53 and mTOR/AKT promote an in vivo switch from apoptosis to senescence upon telomere shortening in zebrafish.
Application : WB
Reactivity : Zebrafish
Ren F et al. Toxicol Appl Pharmacol 2020; 398:115029 Resveratrol protects against PM2.5-induced heart defects in zebrafish embryos as an antioxidant rather than as an AHR antagonist.
Application : IHC-Wm
Reactivity : Zebrafish
Xie H et al. PLoS Genet 2020; 16 (3):e1008655 E2f5 is a versatile transcriptional activator required for spermatogenesis and multiciliated cell differentiation in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Oh CK et al. Hematol Oncol 2020; (Epub) FAM213A is linked to prognostic significance in acute myeloid leukemia through regulation of oxidative stress and myelopoiesis.
Application : IHC-Wm
Reactivity : Zebrafish
Adriana Petrovici et al. J. Mar. Sci. Eng. 2020; 8 (2):73 Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers.
Application : IHC-P
Reactivity : Zebrafish
Murat El Houdigui S et al. Sci Rep 2019; 9 (1):20241 A systems biology approach reveals neuronal and muscle developmental defects after chronic exposure to ionising radiation in zebrafish.
Honjo Y et al. Cell Biol Int 2019; 43 (5):516-527 Cellular responses to ionizing radiation change quickly over time during early development in zebrafish.
Zada D et al. Nat Commun 2019; 10 (1):895 Sleep increases chromosome dynamics to enable reduction of accumulating DNA damage in single neurons.
Li G et al. Front Mol Neurosci 2019; 12:126 Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Zebrafish Development.
Singh AN et al. EMBO J 2019; 38 (21):e102361 The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.
HR Kim et al. bioRxiv 2019; (Epub) The investigation of the role of VHL-HIF signaling in DNA repair and apoptosis in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
He J et al. Hepatology 2019; (Epub) mTORC1 Signaling is Required for the Dedifferentiation from Biliary Cell to Bi-potential Progenitor Cell in Zebrafish Liver Regeneration.
Application : IHC-Wm
Reactivity : Zebrafish
Laura E. Schultz-Rogers et al. bioRxiv 2018; (Epub) Retinoblastoma binding protein 4 maintains cycling neural stem cells and prevents DNA damage and Tp53-dependent apoptosis in rb1 mutant neural progenitors.
Application : IHC-Wm
Reactivity : Zebrafish
ZekunWang et al. BBRC 2018; 505 (2):378-384 Loss of Leucyl-tRNA synthetase b leads to ILFS1-like symptoms in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Gonzalez-Rosa JM et al. Dev Cell 2018; 44 (4):433-446.e7 Myocardial Polyploidization Creates a Barrier to Heart Regeneration in Zebrafish.
Application : IHC-P
Reactivity : Zebrafish
Sorrells S et al. Dis Model Mech 2018; (Epub) Spliceosomal components protect embryonic neurons from R-loop-mediated DNA damage and apoptosis.
Application : ICC/IF
Reactivity : Zebrafish
Kroeger PT Jr et al. Dev Biol 2017; (Epub) The zebrafish kidney mutant zeppelin reveals that brca2/fancd1 is essential for pronephros development.
Application : IHC-Wm
Cao X et al. Proc Natl Acad Sci U S A 2017; 114 (4):687-692 Acetylation promotes TyrRS nuclear translocation to prevent oxidative damage.
Application : IHC-Wm
Reactivity : Zebrafish
Chun C et al. Cell Cycle 2016; 15 (7):957-62 The homologous recombination component EEPD1 is required for genome stability in response to developmental stress of vertebrate embryogenesis.
Application : IHC-Fr
Reactivity : Zebrafish
Zhou R et al. Mutat Res 2016; 793-794:41-50 Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos.
Application : WB
Reactivity : Zebrafish
Carneiro MC et al. PLoS Genet 2016; 12 (1):e1005798 Short Telomeres in Key Tissues Initiate Local and Systemic Aging in Zebrafish.
Application : IHC-P
Reactivity : Zebrafish
Bednarek D et al. Cell Rep 2015; 12 (10):1691-703 Telomerase Is Essential for Zebrafish Heart Regeneration.
Application : WB, IHC-P
Reactivity : Zebrafish
Sasaki T et al. PLoS Genet 2014; 10 (6):e1004409 Aberrant Autolysosomal Regulation Is Linked to The Induction of Embryonic Senescence: Differential Roles of Beclin 1 and p53 in Vertebrate Spns1 Deficiency.
Application : IHC-Wm
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169