Histone H2A.Z (Acetyl Lys4) antibody [RM221]

Cat. No. GTX33591

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM221
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human
Package	100 μg ($449)

See all Histone H2A.Z products

Datasheet

View product citations for antibody GTX33591 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 μg/mL - 2 μg/mL
ICC/IF	1 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Product Note

This antibody reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys4).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family member Z,H2A.Z1,H2A.z,H2A/z,H2AFZ,H2AZ,Histone H2A.Z,H2A.Z variant histone 1

Cellular Localization

Nucleus,Chromosome

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3015 H2AZ1
UniProt: P0C0S5 H2AZ1

Research Area

DATA IMAGES

GTX33591 Image

The GTX33591 reacts to Histone H2A.Z acetylatedat Lysine 4 (K4ac). No cross reactivity with non-modifiedLysine 4 or other acetylated Lysines in histone H2A.

GTX33591 WB Image

WB analysis of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate using GTX33591 Histone H2A.Z (Acetyl Lys4) antibody [RM221].
Dilution : 0.5μg/ml

GTX33591 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX33591 Histone H2A.Z (Acetyl Lys4) antibody [RM221].
Red : Primary antibody
Green : Actin

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty