GeneTex
United States (US)

Histone H2A.Z (Acetyl Lys7) antibody [RM222]

GTX33592_20160517_WB_18121410_670.jpg
GTX33592_20160517_ELISA_18121410_814.jpg

Cat No. GTX33592

Host Rabbit
Clonality Monoclonal
Clone Name RM222
Isotype IgG
Application WB, ELISA, Multiplexing
Reactivity Human
APPLICATION

Application Note

Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.05 ug/mL – 0.5 ug/mL.

Calculated MW

14 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys7)

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H2A Histone Family Member Z,H2A.Z-1,H2A.Z,H2A/Z,H2Az,H2Afz

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX33592_20160517_WB_18121410_670.jpg

GTX33592 WB Image

Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate,using RM222 at 0.5 ug/mL, showed a band ofhistone H2A.Z acetylated at Lysine 7 in treatedHeLa.

GTX33592_20160517_ELISA_18121410_814.jpg

GTX33592 ELISA Image

RM222 specifically reacts to Histone H2A.Z acetylatedat Lysine 7 (K7ac). No cross reactivity with non-modifiedLysine 7 or other acetylated Lysines in histone H2A.

Package List Price ($)
$ 349