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Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade

Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60813
Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60813
Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in ELISA (ELISA). GTX60813
Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in Western Blot (WB). GTX60813
Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in Dot blot (Dot). GTX60813
Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60813

Cat. No. GTX60813

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot, ELISA, ChIP assay, ChIP-seq

Reactivity

 Human, Mouse
Package
50 μg ($549)
See all Histone H2A.Z products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Dot Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
ChIP-seq Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H2A.Z containing the acetylated lysines 4, 7 and 11, using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family member Z , H2A histone family member V , H2A.Z-1 , H2A.z , H2A/z , H2AZ , H2A.Z-2 , H2AV

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60813

GTX60813 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60813

GTX60813 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes CCT5 and EIF4A2, used as positive controls, and for the coding region of the inactive MYT1 gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in ELISA (ELISA). GTX60813

GTX60813 ELISA Image

ELISA analysis of peptide containing the histone modifications of interest using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade.

Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in Western Blot (WB). GTX60813

GTX60813 WB Image

WB analysis of whole cell extracts (25 μg, lane 1) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade.
Dilution : 1:1,000

Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in Dot blot (Dot). GTX60813

GTX60813 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing different multiple acetylations and the unmodified H2A.Z using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade.
Dilution : 1:20,000

Anti-Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60813

GTX60813 ChIP assay Image

ChIP analysis of sheared chromatin from 1x10⁵ K562 cells using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade. The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1.5 Mb region of the X-chromosome (figure 2A and B) and in two regions surrounding the EIF4A2 and CCT5 positive control genes, respectively (figure 2C and D).
Antibody amount : 1μg

REFERENCE

Application Reference

Lorna A Farrelly et al. Nat Commun 2022; 13 (1) : 2195 Chromatin profiling in human neurons reveals aberrant roles for histone acetylation and BET family proteins in schizophrenia.
Application : ChIP-seq
Submit a Reference

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Package List Price ($)
$ 549