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Histone H2A.Z antibody - ChIP grade

Anti-Histone H2A.Z antibody - ChIP grade used in ELISA (ELISA). GTX60812
Anti-Histone H2A.Z antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60812
Anti-Histone H2A.Z antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60812
Anti-Histone H2A.Z antibody - ChIP grade used in Western Blot (WB). GTX60812
Anti-Histone H2A.Z antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60812

Cat. No. GTX60812

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, ChIP assay

Reactivity

 Human
Package
50 μg ($529)
See all Histone H2A.Z products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:500
ELISA 1:5,000
ChIP assay 0.5-5 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antibody was raised in rabbit against histone variant H2A.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family member Z , H2A histone family member V , H2A.Z-1 , H2A.z , H2A/z , H2AZ , H2A.Z-2 , H2AV

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-Histone H2A.Z antibody - ChIP grade used in ELISA (ELISA). GTX60812

GTX60812 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60812 Histone H2A.Z antibody - ChIP grade.

Anti-Histone H2A.Z antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60812

GTX60812 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60812 Histone H2A.Z antibody - ChIP grade. Staining of the cells with the H2A.Z antibody after incubation of the antibody with 10 ng/μl of the immunogen peptide (figure 5B) and with a peptide containing a sequence from the central part of the H2A.Z protein (figure 5C).
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

Anti-Histone H2A.Z antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60812

GTX60812 ChIP assay Image

ChIP analysis of sheared chromatin from 1x10⁵ K562 cells using GTX60812 Histone H2A.Z antibody - ChIP grade. The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome (hg19) using the ELAND algorithm. Figure 2 shows the peak distribution in four genomic regions including the regions surrounding the EIF2S3 and c-fos positive control genes on chromosome X and 14, respectively (figure 2A and B).
Antibody amount : 0.5μg

Anti-Histone H2A.Z antibody - ChIP grade used in Western Blot (WB). GTX60812

GTX60812 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 5, 6, 7 and 8, respectively) using GTX60812 Histone H2A.Z antibody - ChIP grade.
Dilution : 1:1,000

Anti-Histone H2A.Z antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60812

GTX60812 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60812 Histone H2A.Z antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes c-fos and EIF2S3, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

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Package List Price ($)
$ 529