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Histone H2A.Z antibody - VetSignal

Anti-Histone H2A.Z antibody - VetSignal used in Western Blot (WB). GTX135024
Anti-Histone H2A.Z antibody - VetSignal used in Western Blot (WB). GTX135024
Anti-Histone H2A.Z antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135024
Anti-Histone H2A.Z antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135024
Anti-Histone H2A.Z antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135024

Cat No. GTX135024

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Cat, Dog
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to dog Histone H2A.Z.

Purification

Purified by antigen-affinity chromatography.
Glycerol|Glycine|Thimerosal|Tris

Conjugation

Unconjugated

RRID

AB_2887429

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H2A histone family, member Z

Database

Research Area

DATA IMAGES

Anti-Histone H2A.Z antibody - VetSignal used in Western Blot (WB). GTX135024

GTX135024 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.Z antibody (GTX135024) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.Z antibody - VetSignal used in Western Blot (WB). GTX135024

GTX135024 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.Z antibody (GTX135024) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.Z antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135024

GTX135024 IHC-P Image

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat spleen.
Histone H2A.Z stained by Histone H2A.Z antibody (GTX135024) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.Z antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX135024

GTX135024 IHC-P Image

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
Histone H2A.Z stained by Histone H2A.Z antibody (GTX135024) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.Z antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135024

GTX135024 ICC/IF Image

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2A.Z stained by Histone H2A.Z antibody (GTX135024) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

REFERENCE

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379