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Histone H2A.Z antibody

Anti-Histone H2A.Z antibody used in ChIP assay (ChIP assay). GTX108273
Anti-Histone H2A.Z antibody used in Western Blot (WB). GTX108273
Anti-Histone H2A.Z antibody used in Immunoprecipitation (IP). GTX108273
Anti-Histone H2A.Z antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108273
Anti-Histone H2A.Z antibody used in IHC (Paraffin sections) (IHC-P). GTX108273
Anti-Histone H2A.Z antibody used in Western Blot (WB). GTX108273
Anti-Histone H2A.Z antibody used in IHC (Whole mount) (IHC-Wm). GTX108273
Anti-Histone H2A.Z antibody used in Western Blot (WB). GTX108273
Anti-Histone H2A.Z antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108273
Anti-Histone H2A.Z antibody used in IHC (Paraffin sections) (IHC-P). GTX108273
Anti-Histone H2A.Z antibody used in IHC (Paraffin sections) (IHC-P). GTX108273

Cat. No. GTX108273

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay

Reactivity

 Human, Mouse, Zebrafish, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Histone H2A.Z products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Molt-4 , Raji

Predict Reactivity

Rat, Sheep, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.Z. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950474

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family member Z , H2A.Z-1 , H2A.z , H2A/z , H2AZ

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histone H2A.Z antibody used in ChIP assay (ChIP assay). GTX108273

GTX108273 ChIP assay Image

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Histone H2A.Z antibody (GTX108273) The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.

Anti-Histone H2A.Z antibody used in Western Blot (WB). GTX108273

GTX108273 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
B:Raji(GTX27908)
12% SDS PAGE
GTX108273 diluted at 1:3000

Anti-Histone H2A.Z antibody used in Immunoprecipitation (IP). GTX108273

GTX108273 IP Image

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H2A.Z protein by 2 μg of Histone H2A.Z antibody (GTX108273) 15% SDS-PAGE The immunoprecipitated Histone H2A.Z protein was detected by Histone H2A.Z antibody (GTX108273) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Histone H2A.Z antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108273

GTX108273 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Histone H2A.Z(GTX108273) antibody at 1:500 dilution.

Anti-Histone H2A.Z antibody used in IHC (Paraffin sections) (IHC-P). GTX108273

GTX108273 IHC-P Image

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Histone H2A.Z antibody (GTX108273) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A.Z antibody used in Western Blot (WB). GTX108273

GTX108273 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.Z antibody (GTX108273) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.Z antibody used in IHC (Whole mount) (IHC-Wm). GTX108273

GTX108273 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Histone H2A.Z antibody (GTX108273) at 1:200 dilution.

Anti-Histone H2A.Z antibody used in Western Blot (WB). GTX108273

GTX108273 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.Z antibody (GTX108273) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2A.Z antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108273

GTX108273 ICC/IF Image

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2A.Z stained by Histone H2A.Z antibody (GTX108273) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Histone H2A.Z antibody used in IHC (Paraffin sections) (IHC-P). GTX108273

GTX108273 IHC-P Image

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat spleen.
Histone H2A.Z stained by Histone H2A.Z antibody (GTX108273) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2A.Z antibody used in IHC (Paraffin sections) (IHC-P). GTX108273

GTX108273 IHC-P Image

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
Histone H2A.Z stained by Histone H2A.Z antibody (GTX108273) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169