GeneTex
United States (US)

Histone H2A.Z antibody

GTX108273_39757_ChIP_19011507_507.jpg
GTX108273_WB_19011507_194.jpg
GTX108273_39757_IFA_19011507_811.jpg
GTX108273_39757_IP_19011507_192.jpg
GTX108273_39757_20141219_IHC_M_19011507_585.jpg

Cat No. GTX108273

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Molt-4 , Raji

Predict Reactivity

Rat, Sheep, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Histone H2A.Z. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H2A Histone Family Member Z,H2A.Z-1,H2A.Z,H2A/Z,H2Az,H2Afz

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq]

Database

DATA IMAGES
GTX108273_39757_ChIP_19011507_507.jpg

GTX108273 ChIP assay Image

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Histone H2A.Z antibody (GTX108273) The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.

GTX108273_WB_19011507_194.jpg

GTX108273 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
B:Raji(GTX27908)
12% SDS PAGE
GTX108273 diluted at 1:3000

GTX108273_39757_IFA_19011507_811.jpg

GTX108273 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Histone H2A.Z(GTX108273) antibody at 1:500 dilution.

GTX108273_39757_IP_19011507_192.jpg

GTX108273 IP Image

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H2A.Z protein by 2 μg of Histone H2A.Z antibody (GTX108273) 15% SDS-PAGE The immunoprecipitated Histone H2A.Z protein was detected by Histone H2A.Z antibody (GTX108273) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX108273_39757_20141219_IHC_M_19011507_585.jpg

GTX108273 IHC-P Image

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Histone H2A.Z antibody (GTX108273) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169