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Histone H2A.Z antibody

Anti-Histone H2A.Z antibody used in Western Blot (WB). GTX108298
Anti-Histone H2A.Z antibody used in IHC (Paraffin sections) (IHC-P). GTX108298
Anti-Histone H2A.Z antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108298
Anti-Histone H2A.Z antibody used in IHC (Whole mount) (IHC-Wm). GTX108298

Cat. No. GTX108298

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Histone H2A.Z products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2A.Z. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950475

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H2A histone family member Z , H2A.Z-1 , H2A.z , H2A/z , H2AZ

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histone H2A.Z antibody used in Western Blot (WB). GTX108298

GTX108298 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
15% SDS PAGE
GTX108298 diluted at 1:1000

Anti-Histone H2A.Z antibody used in IHC (Paraffin sections) (IHC-P). GTX108298

GTX108298 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using Histone H2A.Z(GTX108298) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A.Z antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108298

GTX108298 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using Histone H2A.Z(GTX108298) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-Histone H2A.Z antibody used in IHC (Whole mount) (IHC-Wm). GTX108298

GTX108298 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Histone H2A.Z antibody (GTX108298) at 1:200 dilution.

REFERENCE

Application Reference

Piquet S et al. Mol Cell 2018; 72 (5) : 888-901.e7 The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169