Histone H2A antibody - ChIP grade

Cat. No. GTX60347

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA, ChIP assay
Reactivity	Human
Package	50 μg ($429)

See all Histone H2A products

Datasheet

View product citations for antibody GTX60347 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2,000
ICC/IF	1:500
ELISA	1:100-1:1,000
ChIP assay	1-2 μg

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminal part of the protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60347 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60347 Histone H2A antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and EIF4A2 promoters, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

GTX60347 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60347 Histone H2A antibody - ChIP grade.
Dilution : 1:2,000

GTX60347 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60347 Histone H2A antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

GTX60347 ELISA Image

ELISA analysis of peptides used for immunization using GTX60347 Histone H2A antibody - ChIP grade.

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Package

List Price ($)

50 μg

$ 429

Qty

Histone H2A antibody - ChIP grade

Cat. No. GTX60347

Host

Clonality

Isotype

Application

Reactivity