Western Blot: 0.5 ug/mL – 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.2 ug/mL – 1 ug/mL.
This antibody reacts to the Histone H2A protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
A peptide corresponding to the C-terminus of human Histone H2A
Protein A affinity purified from an animal origin-free culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
HIST1H2AE Antibody , H2AFA Antibody , H2A.2 Antibody , H2A/a Antibody , H2A.1 Antibody , histone cluster 1, H2ae Antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
GTX33618 WB Image
Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM225 at 0.5 ug/mL
GTX33618 ICC/IF Image
Immunocytochemistry of HeLa cells, using Anti-Histone H2A Rabbit mAb RM225 (red). Actin filaments have been labeled with fluorescein phalloidin (green).