GeneTex
  • Country / Location Selection

United States (US)

Histone H2A antibody [RM225]

Anti-Histone H2A antibody [RM225] used in Western Blot (WB). GTX33618
Anti-Histone H2A antibody [RM225] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33618

Cat No. GTX33618

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM225

Isotype

IgG

Application

WB, ICC/IF, ELISA, Multiplexing

Reactivity

Human
Package
100 μg ($349)

APPLICATION

Application Note

Western Blot: 0.5 ug/mL – 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.2 ug/mL – 1 ug/mL.

Specificity/Sensitivity

This antibody reacts to the Histone H2A protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of human Histone H2A

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HIST1H2AE Antibody , H2AFA Antibody , H2A.2 Antibody , H2A/a Antibody , H2A.1 Antibody , histone cluster 1, H2ae Antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES

Anti-Histone H2A antibody [RM225] used in Western Blot (WB). GTX33618

GTX33618 WB Image

Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM225 at 0.5 ug/mL

Anti-Histone H2A antibody [RM225] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33618

GTX33618 ICC/IF Image

Immunocytochemistry of HeLa cells, using Anti-Histone H2A Rabbit mAb RM225 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

REFERENCE

There are currently no references for Histone H2A antibody [RM225] (GTX33618). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Histone H2A antibody [RM225] (GTX33618). Be the first to share your experience with this product.
Package List Price ($)
$ 349