Histone H2A antibody

Name: Histone H2A antibody
Brand: GeneTex
SKU: GTX129418
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-Histone H2A antibody used in IHC (Paraffin sections) (IHC-P). GTX129418

Anti-Histone H2A antibody used in Western Blot (WB). GTX129418

Cat. No. GTX129418

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Histone H2A products

View product citations for antibody GTX129418 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Histone H2A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885986

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX129418 IHC-P Image

Histone H2A antibody detects Histone H2A protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H2A antibody (GTX129418) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX129418 IHC-P Image

Histone H2A antibody detects Histone H2A protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H2A antibody (GTX129418) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX129418 IHC-P Image

Histone H2A antibody detects Histone H2A protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H2A antibody (GTX129418) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX129418 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A antibody (GTX129418) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129418 WB Image

The data was published in the journal Front Mol Neurosci in 2017.PMID: 28588451

REFERENCE

Application Reference

Alison A Chomiak et al. iScience 2022; 25 (6) : 104354 Nde1 is required for heterochromatin compaction and stability in neocortical neurons.

Yan Guo et al. iScience 2022; 25 (7) : 104519 Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration.

Application : WB, ICC/IF

Reactivity : Mouse

Ho CH et al. Front Physiol 2021; 12 : 725172 α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct Cells.

Application : WB

Wang Y et al. Cell Death Dis. 2021; 12 (2) : 202 Distinct Ring1b complexes defined by DEAD-box helicases and EMT transcription factors synergistically enhance E-cadherin silencing in breast cancer.

Application : WB

Reactivity : Human

Romero A et al. Dev Comp Immunol 2019; 106 : 103585 Extracellular traps (ETosis) can be activated through NADPH-dependent and -independent mechanisms in bivalve mollusks.

Application : ICC/IF

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