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Histone H2A antibody

Anti-Histone H2A antibody used in IHC (Paraffin sections) (IHC-P). GTX129418
Anti-Histone H2A antibody used in Western Blot (WB). GTX129418
Anti-Histone H2A antibody used in IHC (Paraffin sections) (IHC-P). GTX129418
Anti-Histone H2A antibody used in IHC (Paraffin sections) (IHC-P). GTX129418
Anti-Histone H2A antibody used in IHC (Paraffin sections) (IHC-P). GTX129418

Cat No. GTX129418

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Histone H2A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H2A.1 Antibody , histone cluster 1, H2ae Antibody , HIST1H2AE Antibody , H2AFA Antibody , H2A.2 Antibody , H2A/a Antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Research Area

DATA IMAGES
Anti-Histone H2A antibody used in IHC (Paraffin sections) (IHC-P). GTX129418

GTX129418 IHC-P Image

Histone H2A antibody detects Histone H2A protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H2A antibody (GTX129418) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A antibody used in Western Blot (WB). GTX129418

GTX129418 WB Image

Histone H2A antibody detects Histone H2A protein by western blot analysis.
A. 30 μg MCF-7 whole cell extract
B. 30 μg MDA-MB-231 whole cell extract
15 % SDS-PAGE
Histone H2A antibody (GTX129418) dilution: 1:10000

Anti-Histone H2A antibody used in IHC (Paraffin sections) (IHC-P). GTX129418

GTX129418 IHC-P Image

Histone H2A antibody detects Histone H2A protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H2A antibody (GTX129418) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A antibody used in IHC (Paraffin sections) (IHC-P). GTX129418

GTX129418 IHC-P Image

Histone H2A antibody detects Histone H2A protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H2A antibody (GTX129418) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H2A antibody used in IHC (Paraffin sections) (IHC-P). GTX129418

GTX129418 IHC-P Image

Histone H2A antibody detects Histone H2A protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H2A antibody (GTX129418) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

Histone H2A antibody Cat No. GTX129418

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Sep-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : mpkCCD cells
Amount used : 4 μg
Blocking : 0.1%BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:2500, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169