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Histone H2A type 1H antibody, N-term

Anti-Histone H2A type 1H antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81004
Anti-Histone H2A type 1H antibody, N-term used in Western Blot (WB). GTX81004
Anti-Histone H2A type 1H antibody, N-term used in Flow cytometry (FACS). GTX81004

Cat. No. GTX81004

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all Histone H2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2AH.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11164520

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone cluster 1 H2A family member h , H2A/S , H2AFALii , H2AH , dJ86C11.1

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H2A type 1H antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81004

GTX81004 IHC-P Image

IHC-P analysis of human brain tissue using GTX81004 Histone H2A type 1H antibody, N-term.

Anti-Histone H2A type 1H antibody, N-term used in Western Blot (WB). GTX81004

GTX81004 WB Image

WB analysis of A2058, A375, K562, HeLa, MCF-7 cell lysate (35ug/lane) using GTX81004 Histone H2A type 1H antibody, N-term.

Anti-Histone H2A type 1H antibody, N-term used in Flow cytometry (FACS). GTX81004

GTX81004 FACS Image

FACS analysis of MCF-7 cells using GTX81004 Histone H2A type 1H antibody, N-term.
Green : primary antibody
Blue : negative control

REFERENCE

Application Reference

Wang M et al. Pathol Oncol Res 2018; (Epub) Quantitative Proteomics Identify the Possible Tumor Suppressive Role of Protease-Activated Receptor-4 in Esophageal Squamous Cell Carcinoma Cells.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399