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Histone H2A.vS137ph (phospho Ser137) antibody

Anti-Histone H2A.vS137ph (phospho Ser137) antibody used in Western Blot (WB). GTX48733

Cat No. GTX48733

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, IHC

Reactivity

Drosophila
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:400 - 1:1600
ELISA 1:5000 - 1:20000
IHC 2 ug/ml
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein.

Purification

Affinity purified using phosphopeptide and cross-adsorbed against non-phosphorylated peptide

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Histone H2A variant , 5499 , CG5499 , Dmel\CG5499 , H2A , H2A.F/Z , H2A.V , H2A.X , H2A.Z , H2AV , H2AV_DROM , H2AX , H2AZ , H2Av , H2AvD , H2a.V , H2av , H2avD , HIS , HIS2AV , HIS2AVD , His , His2 , His2A , His2AV , His2AvD , HisH2Av , Hist , Hist2av , gamma-H2AV , gamma-H2Av , gamma-HIS2AV , gamma-His2Av , gammaH2AV , gammaH2Av , h2AvD , his , his2Av , l(3)05146 , l(3)810 , l(3)97Dd , l(3)L1602

Cellular Localization

Nucleus

Background

Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX "knock out" mice have an increased incidence of cancer.

Database

Research Area

DATA IMAGES

Anti-Histone H2A.vS137ph (phospho Ser137) antibody used in Western Blot (WB). GTX48733

GTX48733 WB Image

Western blot using GeneTex's affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf