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Histone H2AK5ac (acetyl Lys5) antibody [GT1262]

Orthogonal
Anti-Histone H2AK5ac (acetyl Lys5) antibody [GT1262] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633388
Anti-Histone H2AK5ac (acetyl Lys5) antibody [GT1262] used in Western Blot (WB). GTX633388
Anti-Histone H2AK5ac (acetyl Lys5) antibody [GT1262] used in IHC (Paraffin sections) (IHC-P). GTX633388
Anti-Histone H2AK5ac (acetyl Lys5) antibody [GT1262] used in IHC (Paraffin sections) (IHC-P). GTX633388

Cat. No. GTX633388

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1262

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Histone H2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

HeLa , HeLa (0.4 μM Trichoststin A for 18 hr)

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5 of human Histone H2A. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888337

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H2AK5ac (acetyl Lys5) antibody [GT1262] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633388

GTX633388 ICC/IF Image

Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody [GT1262] (GTX633388) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102078) diluted at 1:300.
Scale bar = 10 μm.

Anti-Histone H2AK5ac (acetyl Lys5) antibody [GT1262] used in Western Blot (WB). GTX633388

GTX633388 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2AK5ac (acetyl Lys5) antibody [GT1262] (GTX633388) diluted at 1:1000.

Anti-Histone H2AK5ac (acetyl Lys5) antibody [GT1262] used in IHC (Paraffin sections) (IHC-P). GTX633388

GTX633388 IHC-P Image

Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] (GTX633388) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2AK5ac (acetyl Lys5) antibody [GT1262] used in IHC (Paraffin sections) (IHC-P). GTX633388

GTX633388 IHC-P Image

Histone H2AK5ac (acetyl Lys5) antibody [GT1262] detects Histone H2AK5ac (acetyl Lys5) protein at nucleus in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Histone H2AK5ac (acetyl Lys5) antibody [GT1262] (GTX633388) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Huang FI et al. Front Oncol 2019; 9 : 249 MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells.
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations