GeneTex
  • Country / Location Selection

United States (US)

Histone H2AK5ac (acetyl Lys5) antibody

Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Western Blot (WB). GTX128961
Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Dot blot (Dot). GTX128961
Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Immunoprecipitation (IP). GTX128961
Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128961

Cat No. GTX128961

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, Dot

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IP 1:100-1:10000
Dot Assay dependent
Not tested in other applications.

Positive Control

HeLa

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2AK5ac (acetyl Lys5). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H2a-615 Antibody , H2A/q Antibody , H2A Antibody , HIST2H2AA3 Antibody , histone cluster 2 , H2aa3 Antibody , H2AFO Antibody , H2A/O Antibody , HIST2H2AA Antibody , H2A.2 Antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Western Blot (WB). GTX128961

GTX128961 WB Image

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) dilution: 1:10000

Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Dot blot (Dot). GTX128961

GTX128961 Dot Image

Dotblot analysis of anti-Histone H2AK5ac (acetyl Lys5) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 1:5000 dilution.
A: Peptide samples of H2A.2K5 un
B: Peptide samples of H2A.2K5ac
C: Peptide samples of H2BK20 un
D: Peptide samples of H2BK20ac
E: Peptide samples of H2BK5 un
F: Peptide samples of H2BK5ac

Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Immunoprecipitation (IP). GTX128961

GTX128961 IP Image

Histone H2AK5ac (acetyl Lys5) antibody immunoprecipitates Histone H2AK5ac (acetyl Lys5) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H2AK5ac (acetyl Lys5) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2AK5ac (acetyl Lys5) protein by 2.5 μg of Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 12% SDS-PAGE The immunoprecipitated Histone H2AK5ac (acetyl Lys5) protein was detected by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1 : 10000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H2AK5ac (acetyl Lys5) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128961

GTX128961 ICC/IF Image

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 0.4 μM TSA for 18 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

There are currently no references for Histone H2AK5ac (acetyl Lys5) antibody (GTX128961). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Histone H2AK5ac (acetyl Lys5) antibody (GTX128961). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169