Histone H2B (Acetyl Lys20) antibody [RM235]

Cat No. GTX33593

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM235
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human
Package	100 μg ($399)

See all Histone H2B products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.5 μg/mL - 2 μg/mL
ICC/IF	1 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL

Not tested in other applications.

Specificity/Sensitivity

This antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.09% sodium azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX33593 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX33593 Histone H2B (Acetyl Lys20) antibody [RM235].
Red : Primary antibody
Green : Actin

GTX33593 WB Image

WB analysis of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate using GTX33593 Histone H2B (Acetyl Lys20) antibody [RM235].
Dilution : 0.5μg/ml

GTX33593 Image

The GTX33593 reacts to Histone H2B acetylatedat Lysine 20 (K20ac). No cross reactivity with non- modified Lysine 20 or other acetylated Lysines in histone H2B.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty

Histone H2B (Acetyl Lys20) antibody [RM235]

Cat No. GTX33593

Host

Clonality

Clone Name

Isotype

Application

Reactivity