GeneTex
United States (US)

Histone H2B (Acetyl Lys20) antibody [RM235]

GTX33593_20160517_WB_18121410_550.jpg
GTX33593_20160517_ELISA_18121410_678.jpg

Cat No. GTX33593

Host Rabbit
Clonality Monoclonal
Clone Name RM235
Isotype IgG
Application WB, ELISA, Multiplexing
Reactivity Human
APPLICATION

Application Note

Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Specificity/Sensitivity

This antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20).

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX33593_20160517_WB_18121410_550.jpg

GTX33593 WB Image

Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate,using RM235 at 0.5 ug/mL.

GTX33593_20160517_ELISA_18121410_678.jpg

GTX33593 ELISA Image

RM235 specifically reacts to Histone H2B acetylatedat Lysine 20 (K20ac). No cross reactivity with non- modified Lysine 20 or other acetylated Lysines in histone H2B.

Package List Price ($)
$ 349