ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60346 Histone H2B antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis.

ELISA analysis of peptides used for immunization using GTX60346 Histone H2B antibody - ChIP grade.

WB analysis of whole cell extracts from HeLa cells (40 μg, lane 1) and on 1 μg of recombinant histone H2B (lane 2) using GTX60346 Histone H2B antibody - ChIP grade.
Dilution : 1:10,000

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60346 Histone H2B antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000