GeneTex

  • Country / Location Selection

United States (US)

Histone H2B antibody - ChIP grade

Anti-Histone H2B antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60346
Anti-Histone H2B antibody - ChIP grade used in Western Blot (WB). GTX60346
Anti-Histone H2B antibody - ChIP grade used in ELISA (ELISA). GTX60346
Anti-Histone H2B antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60346

Cat. No. GTX60346

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, ChIP assay

Reactivity

 Human
Package
50 μg ($429)
See all Histone H2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,000 - 1/10,000
ICC/IF 1:1,000
ELISA 1:1,000 - 1/5,000
ChIP assay 1-5 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminal part of the protein.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H2B antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60346

GTX60346 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60346 Histone H2B antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000

Anti-Histone H2B antibody - ChIP grade used in Western Blot (WB). GTX60346

GTX60346 WB Image

WB analysis of whole cell extracts from HeLa cells (40 μg, lane 1) and on 1 μg of recombinant histone H2B (lane 2) using GTX60346 Histone H2B antibody - ChIP grade.
Dilution : 1:10,000

Anti-Histone H2B antibody - ChIP grade used in ELISA (ELISA). GTX60346

GTX60346 ELISA Image

ELISA analysis of peptides used for immunization using GTX60346 Histone H2B antibody - ChIP grade.

Anti-Histone H2B antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60346

GTX60346 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60346 Histone H2B antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as negative controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis.

REFERENCE

There are currently no references for Histone H2B antibody - ChIP grade (GTX60346). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Histone H2B antibody - ChIP grade (GTX60346). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 429