GeneTex
United States (US)

Histone H2B antibody [RM230]

GTX33619_20160517_WB_18121410_952.jpg
GTX33619_20160517_ICCIF_18121410_226.jpg

Cat No. GTX33619

Host Rabbit
Clonality Monoclonal
Clone Name RM230
Isotype IgG
Application WB, ICC/IF, ELISA, Multiplexing
Reactivity Human
APPLICATION

Application Note

Western Blot: 0.1 ug/mL – 1 ug/mL, ICC: 0.5 μg/mL - 1 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.2 ug/mL – 1 ug/mL.

Specificity/Sensitivity

This antibody reacts to the Histone H2B protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of human Histone H2B.

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

histone cluster 2, H2be Antibody , GL105 Antibody , H2B.1 Antibody , H2BQ Antibody , H2BGL105 Antibody , HIST2H2BE Antibody , H2BFQ Antibody , H2B Antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES
GTX33619_20160517_WB_18121410_952.jpg

GTX33619 WB Image

Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM230 at 0.2 ug/mL

GTX33619_20160517_ICCIF_18121410_226.jpg

GTX33619 ICC/IF Image

Immunocytochemical staining of HeLa cells, using Anti-Histone H2B Rabbit mAb RM230 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Package List Price ($)
$ 349