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Histone H2B antibody [RM230]

Anti-Histone H2B antibody [RM230] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33619
Anti-Histone H2B antibody [RM230] used in Western Blot (WB). GTX33619

Cat No. GTX33619

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM230

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 μg/mL - 1 μg/mL
ICC/IF 0.5 μg/mL - 1 μg/mL
ELISA 0.2 μg/mL - 1 μg/mL
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to the Histone H2B protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.09% sodium azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of human Histone H2B.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GL105,H2B,H2B.1,H2BFQ,H2BGL105,H2BQ,HIST2H2BE,histone cluster 2 H2B family member e,H2B clustered histone 21

Cellular Localization

Nucleus,Chromosome

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H2B antibody [RM230] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33619

GTX33619 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33619 Histone H2B antibody [RM230].
Red : Primary antibody
Green : Actin

Anti-Histone H2B antibody [RM230] used in Western Blot (WB). GTX33619

GTX33619 WB Image

WB analysis of recombinant histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562 using GTX33619 Histone H2B antibody [RM230].
Dilution : 0.2μg/ml

REFERENCE

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REVIEW

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