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Histone H2B antibody

Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX115955
Anti-Histone H2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115955
Anti-Histone H2B antibody used in Western Blot (WB). GTX115955
Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX115955
Anti-Histone H2B antibody used in Western Blot (WB). GTX115955

Cat No. GTX115955

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Histone H2B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H2B.1B antibody, H2B/b antibody, H2BFB antibody, HIRIP2 antibody, MGC90432 antibody, dJ221C16.6 antibody, HIST1H2BD antibody, "histone 1, H2bd antibody", histone H2B.b antibody, histone H2B type 1-D antibody, HIRA-interacting protein 2 antibody, histone H2B.1 B antibody, "H2B histone family, member B antibody", "histone cluster 1, H2bd antibody"

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Research Area

DATA IMAGES
Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX115955

GTX115955 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Histone H2B(GTX115955) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115955

GTX115955 ICC/IF Image

Histone H2B antibody detects Histone H2B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: Histone H2B protein stained by Histone H2B antibody (GTX115955) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Histone H2B antibody used in Western Blot (WB). GTX115955

GTX115955 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX115955) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX115955

GTX115955 IHC-P Image

Histone H2B antibody detects Histone H2B protein at nucleus in mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Histone H2B antibody (GTX115955) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H2B antibody used in Western Blot (WB). GTX115955

GTX115955 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX115955) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169