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Histone H2B antibody

Anti-Histone H2B antibody used in Western Blot (WB). GTX129434
Anti-Histone H2B antibody used in Western Blot (WB). GTX129434
Anti-Histone H2B antibody used in Western Blot (WB). GTX129434

Cat No. GTX129434

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HeLa , HeLa nuclear extract , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Histone H2B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H2BFQ Antibody , histone cluster 2, H2be Antibody , H2BGL105 Antibody , H2BQ Antibody , GL105 Antibody , H2B Antibody , HIST2H2BE Antibody , H2B.1 Antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-Histone H2B antibody used in Western Blot (WB). GTX129434

GTX129434 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Histone H2B antibody (GTX129434) diluted at 1:500 and competitor's antibody (sc-10808) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Histone H2B antibody used in Western Blot (WB). GTX129434

GTX129434 WB Image

Histone H2B antibody detects Histone H2B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX129434) diluted by 1:1000.

Anti-Histone H2B antibody used in Western Blot (WB). GTX129434

GTX129434 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX129434) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169