Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Histone H2B antibody (GTX129565) diluted at 1:5000 and competitor's antibody (sc-10808) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX129565) diluted at 1:20000.

Immunoprecipitation of Histone H2B protein from HeLa whole cell extracts using 5 μg of Histone H2B antibody (GTX129565).
Western blot analysis was performed using Histone H2B antibody (GTX129565) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.