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Histone H2B antibody

Anti-Histone H2B antibody used in Western Blot (WB). GTX129565
Anti-Histone H2B antibody used in Immunoprecipitation (IP). GTX129565
Anti-Histone H2B antibody used in Western Blot (WB). GTX129565

Cat No. GTX129565

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
IP 1:100-1:500
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886033

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Histone H2B antibody used in Western Blot (WB). GTX129565

GTX129565 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Histone H2B antibody (GTX129565) diluted at 1:5000 and competitor's antibody (sc-10808) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Histone H2B antibody used in Immunoprecipitation (IP). GTX129565

GTX129565 IP Image

Immunoprecipitation of Histone H2B protein from HeLa whole cell extracts using 5 μg of Histone H2B antibody (GTX129565).
Western blot analysis was performed using Histone H2B antibody (GTX129565) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Histone H2B antibody used in Western Blot (WB). GTX129565

GTX129565 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX129565) diluted at 1:20000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf