APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:1000 |
1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Histone H2B.
Purification
Purified by antigen-affinity chromatography
From serum
Purity
> 95% (by SDS-PAGE)
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
histone cluster 1 H2B family member h , H2B/j , H2BFJ
Cellular Localization
Nucleus
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
Database
Research Area
DATA IMAGES
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GTX66686 IHC-P Image
IHC-P analysis of human brain tissue using GTX66686 Histone H2B antibody.
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GTX66686 WB Image
WB analysis of various samples using GTX66686 Histone H2B antibody. Lane1 : Hela whole cell lysate
Lane2 : Raw264.7 whole cell lysate
Lane3 : PC12 whole cell lysate Dilution : 1:500
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REFERENCE
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REVIEW
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