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Histone H2B antibody

Anti-Histone H2B antibody used in Western Blot (WB). GTX66686
Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX66686

Cat No. GTX66686

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to the N-terminal of Human Histone H2B.

Purification

Purified by antigen-affinity chromatography
From serum

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone cluster 1 H2B family member h , H2B/j , H2BFJ

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Database

  • Gene ID: 8345 HIST1H2BH
  • UniProt: Q93079 HIST1H2BH

Research Area

DATA IMAGES

Anti-Histone H2B antibody used in Western Blot (WB). GTX66686

GTX66686 WB Image

WB analysis of various samples using GTX66686 Histone H2B antibody.
Lane1 : Hela whole cell lysate
Lane2 : Raw264.7 whole cell lysate
Lane3 : PC12 whole cell lysate
Dilution : 1:500

Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX66686

GTX66686 IHC-P Image

IHC-P analysis of human brain tissue using GTX66686 Histone H2B antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 349