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Histone H2B antibody

Anti-Histone H2B antibody used in Western Blot (WB). GTX87385
Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX87385
Anti-Histone H2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87385

Cat. No. GTX87385

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Pig, Monkey
Package
100 μg ($399)
See all Histone H2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H2B (8-59).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10725936

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-Histone H2B antibody used in Western Blot (WB). GTX87385

GTX87385 WB Image

WB analysis of COS7 cell, treated with TSA 400nM (24h) lysates using GTX87385 Histone H2B antibody. The lane on the right is blocked with the synthesized peptide.

Anti-Histone H2B antibody used in IHC (Paraffin sections) (IHC-P). GTX87385

GTX87385 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX87385 Histone H2B antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Histone H2B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87385

GTX87385 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX87385 Histone H2B antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Paudyal A et al. J Physiol 2016; 594 (12) : 3287-305 Nuclear accumulation of myocyte muscle LIM protein is regulated by heme oxygenase 1 and correlates with cardiac function in the transition to failure.
Application : WB
Reactivity : Pig
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399