GeneTex
United States (US)

Histone H2B (phospho Ser14) antibody [RM238]

GTX33598_20160517_WB_18121410_765.jpg
GTX33598_20160517_ICCIF_18121410_721.jpg

Cat No. GTX33598

Host Rabbit
Clonality Monoclonal
Clone Name RM238
Isotype IgG
Application WB, ICC/IF, ELISA, Multiplexing
Reactivity Human
APPLICATION

Application Note

Western Blot: 0.5 ug/mL - 2 ug/mL, ICC: 1 μg/mL - 2 μg/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 1 ug/mL.

Specificity/Sensitivity

This antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

A phospho-peptide corresponding to Phospho-Histone H2B (Ser14).

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX33598_20160517_WB_18121410_765.jpg

GTX33598 WB Image

Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM238 at 0.5 ug/mL, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells.

GTX33598_20160517_ICCIF_18121410_721.jpg

GTX33598 ICC/IF Image

Immunocytochemistry of HeLa cells using Anti-Phospho-Histone H2B (Ser14) Rabbit mAb RM238 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).

Package List Price ($)
$ 349