Histone H2B (phospho Ser14) antibody [RM238]

Cat. No. GTX33598

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM238
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human
Package	100 μg ($449)

See all Histone H2B products

Datasheet

View product citations for antibody GTX33598 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 μg/mL - 2 μg/mL
ICC/IF	1 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL

Not tested in other applications.

Product Note

This antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho-peptide corresponding to Phospho-Histone H2B (Ser14).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

GTX33598 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33598 Histone H2B (phospho Ser14) antibody [RM238].
Red : Primary antibody
Green : Actin

GTX33598 WB Image

WB analysis of acid extracts of HeLa cells treated or non-treated with Nocodazole using GTX33598 Histone H2B (phospho Ser14) antibody [RM238].
Dilution : 0.5μg/ml

REFERENCE

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Package

List Price ($)

100 μg

$ 449

Qty

Histone H2B (phospho Ser14) antibody [RM238]

Cat. No. GTX33598

Host

Clonality

Clone Name

Isotype

Application

Reactivity