GeneTex
United States (US)

Histone H2BK15ac (acetyl Lys15) antibody

Histone-H2B-acetyl-Lys15-antibody-GTX88002-WB-1_18121411_717.jpg
Histone-H2B-acetyl-Lys15-antibody-GTX88002-ICC_IF-1_18121411_513.jpg
Histone-H2B-acetyl-Lys15-antibody-GTX88002-IHC-1_18121411_250.jpg

Cat No. GTX88002

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Specificity/Sensitivity

Histone H2B (Acetyl-Lys15) Antibody detects endogenous levels of total Histone H2B protein only when acetylated at lysine15.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys15.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acet

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histone cluster1, Histone cluster 1, member F, H2BFF, histone 1, H2bb, Histone H2B, H2B/f, histone1, H2B histone family, H2B.1, Histone H2B (acetyl K15), Histone H2B (acetyl Lys15), Histone H2B acetyl K15, Histone H2B acetyl Lys15, Acetyl Histone H2B (K15), Acetyl Histone H2B (Lys15)

Research Area

DATA IMAGES
Histone-H2B-acetyl-Lys15-antibody-GTX88002-WB-1_18121411_717.jpg

GTX88002 WB Image

Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B (Acetyl-Lys15) Antibody. The lane on the right is treated with the synthesized peptide.

Histone-H2B-acetyl-Lys15-antibody-GTX88002-ICC_IF-1_18121411_513.jpg

GTX88002 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is treated with the synthesized peptide.

Histone-H2B-acetyl-Lys15-antibody-GTX88002-IHC-1_18121411_250.jpg

GTX88002 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is treated with the synthesized peptide.