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Histone H2BK20ac (acetyl Lys20) antibody [GT578]

Anti-Histone H2BK20ac (acetyl Lys20) antibody [GT578] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633409
Anti-Histone H2BK20ac (acetyl Lys20) antibody [GT578] used in Western Blot (WB). GTX633409

Cat No. GTX633409

Host Mouse
Clonality Monoclonal
Clone Name GT578
Isotype IgG1
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

HeLa (0.4 μM Trichoststin A treatment for 18 hr)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2BK20ac (acetyl Lys20). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Histone H2BK20ac (acetyl Lys20) antibody [GT578] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633409

GTX633409 ICC/IF Image

Histone H2BK20ac (acetyl Lys20) antibody [GT578] detects Histone H2BK20ac (acetyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2BK20ac (acetyl Lys20) stained by Histone H2BK20ac (acetyl Lys20) antibody [GT578] (GTX633409) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10 μm.

Anti-Histone H2BK20ac (acetyl Lys20) antibody [GT578] used in Western Blot (WB). GTX633409

GTX633409 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2BK20ac (acetyl Lys20) antibody [GT578] (GTX633409) diluted at 1:1000.

REFERENCE
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169