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Histone H2BK20ac (acetyl Lys20) antibody

Anti-Histone H2BK20ac (acetyl Lys20) antibody used in Dot blot (Dot). GTX128962
Anti-Histone H2BK20ac (acetyl Lys20) antibody used in Western Blot (WB). GTX128962
Anti-Histone H2BK20ac (acetyl Lys20) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128962
Anti-Histone H2BK20ac (acetyl Lys20) antibody used in Immunoprecipitation (IP). GTX128962

Cat No. GTX128962

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, Dot

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:5000
Dot Assay dependent
Not tested in other applications.

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide  corresponding to human Histone H2BK20ac (acetyl Lys20)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Histone H2BK20ac (acetyl Lys20) antibody used in Dot blot (Dot). GTX128962

GTX128962 Dot Image

Dotblot analysis of anti-Histone H2BK20ac (acetyl Lys20) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) 1:5000 dilution.
A: Peptide samples of H2A.2K5 un
B: Peptide samples of H2A.2K5ac
C: Peptide samples of H2BK20 un
D: Peptide samples of H2BK20ac
E: Peptide samples of H2BK5 un
F: Peptide samples of H2BK5ac

Anti-Histone H2BK20ac (acetyl Lys20) antibody used in Western Blot (WB). GTX128962

GTX128962 WB Image

Histone H2BK20ac (acetyl Lys20) antibody detects Histone H2BK20ac (acetyl Lys20) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) dilution: 1:5000

Anti-Histone H2BK20ac (acetyl Lys20) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128962

GTX128962 ICC/IF Image

Histone H2BK20ac (acetyl Lys20) antibody detects Histone H2BK20ac (acetyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: 0.4M TSA-treated for 18hr HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2BK20ac (acetyl Lys20) protein stained by Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Histone H2BK20ac (acetyl Lys20) antibody used in Immunoprecipitation (IP). GTX128962

GTX128962 IP Image

Histone H2BK20ac (acetyl Lys20) antibody immunoprecipitates Histone H2BK20ac (acetyl Lys20) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H2BK20ac (acetyl Lys20) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2BK20ac (acetyl Lys20) protein by 2.5 μg of Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) 12% SDS-PAGE The immunoprecipitated Histone H2BK20ac (acetyl Lys20) protein was detected by Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169