Histone H2BK20ac (acetyl Lys20) antibody

Cat. No. GTX128962

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP, Dot
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Histone H2B products

Datasheet
Document

View product citations for antibody GTX128962 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IP	1:100-1:5000
Dot	Assay dependent

Not tested in other applications.

Positive Control

HeLa (0.012% DMSO treatment for 18 hr) , HeLa (0.4 μM Trichostatin A treatment for 18 hr)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys20 of human Histone H2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885850

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

GTX128962 ICC/IF Image

Histone H2BK20ac (acetyl Lys20) antibody detects Histone H2BK20ac (acetyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: 0.4M TSA-treated for 18hr HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2BK20ac (acetyl Lys20) protein stained by Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX128962 WB Image

Histone H2BK20ac (acetyl Lys20) antibody detects Histone H2BK20ac (acetyl Lys20) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) dilution: 1:5000

GTX128962 IP Image

Histone H2BK20ac (acetyl Lys20) antibody immunoprecipitates Histone H2BK20ac (acetyl Lys20) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H2BK20ac (acetyl Lys20) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2BK20ac (acetyl Lys20) protein by 2.5 μg of Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) 12% SDS-PAGE The immunoprecipitated Histone H2BK20ac (acetyl Lys20) protein was detected by Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX128962 Dot Image

Dotblot analysis of anti-Histone H2BK20ac (acetyl Lys20) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) 1:5000 dilution.
A: Peptide samples of H2A.2K5 un
B: Peptide samples of H2A.2K5ac
C: Peptide samples of H2BK20 un
D: Peptide samples of H2BK20ac
E: Peptide samples of H2BK5 un
F: Peptide samples of H2BK5ac

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

Histone H2BK20ac (acetyl Lys20) antibody

Cat. No. GTX128962

Host

Clonality

Isotype

Application

Reactivity