Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Dotblot analysis of anti-Histone H2BK20ac (acetyl Lys20) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) 1:5000 dilution.
A: Peptide samples of H2A.2K5 un
B: Peptide samples of H2A.2K5ac
C: Peptide samples of H2BK20 un
D: Peptide samples of H2BK20ac
E: Peptide samples of H2BK5 un
F: Peptide samples of H2BK5ac

Histone H2BK20ac (acetyl Lys20) antibody immunoprecipitates Histone H2BK20ac (acetyl Lys20) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H2BK20ac (acetyl Lys20) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2BK20ac (acetyl Lys20) protein by 2.5 μg of Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) 12% SDS-PAGE The immunoprecipitated Histone H2BK20ac (acetyl Lys20) protein was detected by Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Histone H2BK20ac (acetyl Lys20) antibody detects Histone H2BK20ac (acetyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: 0.4M TSA-treated for 18hr HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2BK20ac (acetyl Lys20) protein stained by Histone H2BK20ac (acetyl Lys20) antibody (GTX128962) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.