Histone H2BK5ac (acetyl Lys5) antibody detects Histone H2BK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis.
Sample: 0.4M TSA-treated for 18hr HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2BK5ac (acetyl Lys5) protein stained by Histone H2BK5ac (acetyl Lys5) antibody (GTX128963) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

Histone H2BK5ac (acetyl Lys5) antibody detects Histone H2BK5ac (acetyl Lys5) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H2BK5ac (acetyl Lys5) antibody (GTX128963) dilution: 1:1000

Dotblot analysis of anti-Histone H2BK5ac (acetyl Lys5) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2BK5ac (acetyl Lys5) antibody (GTX128963) 1:5000 dilution.
A: Peptide samples of H2A.2K5 un
B: Peptide samples of H2A.2K5ac
C: Peptide samples of H2BK20 un
D: Peptide samples of H2BK20ac
E: Peptide samples of H2BK5 un
F: Peptide samples of H2BK5ac