GeneTex
United States (US)

Histone H2BK5ac (acetyl Lys5) antibody

Histone-H2B-acetyl-Lys5-antibody-GTX88015-IHC-1_18122102_779.jpg
Histone-H2B-acetyl-Lys5-antibody-GTX88015-ICC_IF-1_18122102_560.jpg

Cat No. GTX88015

Host Rabbit
Clonality Polyclonal
Application ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:1000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

13 kDa. ( Note )

Specificity/Sensitivity

Histone H2B (Acetyl-Lys5) Antibody detects endogenous levels of total Histone H2B protein only when acetylated at lysine5.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys5.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acet

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Histone cluster1, Histone cluster 1, member F, H2BFF, histone 1, H2bb, Histone H2B, H2B/f, histone1, H2B histone family, H2B.1, Histone H2B (acetyl K5), Histone H2B (acetyl Lys5), Histone H2B acetyl K5, Histone H2B acetyl Lys5, Acetyl Histone H2B (K5), Acetyl Histone H2B (Lys5)

Research Area

DATA IMAGES
Histone-H2B-acetyl-Lys5-antibody-GTX88015-IHC-1_18122102_779.jpg

GTX88015 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.

Histone-H2B-acetyl-Lys5-antibody-GTX88015-ICC_IF-1_18122102_560.jpg

GTX88015 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339