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Histone H3.3 antibody [RM190]

Anti-Histone H3.3 antibody [RM190] used in Western Blot (WB). GTX60889
Anti-Histone H3.3 antibody [RM190] used in  (). GTX60889

Cat No. GTX60889

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM190

Isotype

IgG

Application

WB, ELISA

Reactivity

Human
Package
100 μg ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL - 2 μg/mL
ELISA 0.2 μg/mL - 1 μg/mL
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Product Note

This antibody reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A peptide corresponding to human Histone H3.3.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus,Chromosome

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Histone H3.3 antibody [RM190] used in Western Blot (WB). GTX60889

GTX60889 WB Image

WB analysis of HeLa whole cell, recombinant histone H3.3, and histone H3.1 protein lysates using GTX60889 Histone H3.3 antibody [RM190]. No cross activity with Histone H3.1.
Dilution : 1μg/ml

Anti-Histone H3.3 antibody [RM190] used in  (). GTX60889

GTX60889 Image

The GTX60889 reacts to Histone H3.3. No cross reactivity with Histone H3.1.

REFERENCE

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REVIEW

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Package List Price ($)
$ 419